# Local Delivery of Genistein in Peri-Implant Defects Enhances Osseointegration in Ovariectomized Rats **Authors:** Nathália Dantas Duarte, Fábio Roberto de Souza Batista, Marcelly Braga Gomes, Laura Gabriela Macedo, Naara Gabriela Monteiro, Gabriel Mulinari-Santos, Pedro Henrique Silva Gomes-Ferreira, Paulo Noronha Lisboa-Filho, Luiz Meirelles, Roberta Okamoto PMC · DOI: 10.1007/s00223-026-01496-9 · Calcified Tissue International · 2026-02-17 ## TL;DR Adding genistein to biomaterials improves bone healing around dental implants in rats with estrogen deficiency. ## Contribution Genistein-functionalized biomaterials enhance peri-implant bone repair in ovariectomized rats. ## Key findings - Genistein-functionalized deproteinized bovine bone (BO + GEN) showed the highest removal torque (5.4 Ncm). - Genistein-functionalized materials upregulated bone markers (RANKL, OPG, OCN, IBSP) and increased bone volume percentage (BV/TV). - Trabecular separation was significantly reduced in BO + GEN compared to controls. ## Abstract Estrogen deficiency, a key factor in osteoporosis, may influence peri-implant healing, which requires cautious and individualized planning for dental implant placement. Functionalizing biomaterials with bioactive molecules, such as soy-derived isoflavone, could enhance their osteoconductive potential. Investigate the performance of genistein in the functionalization of bioactive glass and deproteinized bovine bone by sonochemistry on peri-implant bone repair in ovariectomized rats. In total, 50 female rats were divided into five groups (n = 10): blood clot (CLOT); bioactive glass (BG); bioactive glass functionalized with genistein (BG + GEN); deproteinized bovine bone (BO); and deproteinized bovine bone functionalized with genistein (BO + GEN). Thirty days after ovariectomy, implants were placed in the tibia of the rats. Calcein and alizarin were administered at 14 and 24 days post-surgery, respectively. The rats were euthanized 28 days after implant placement for analysis. BO + GEN showed the highest removal torque (5.4 Ncm), significantly higher than BO (2.3 Ncm; p < 0.05), followed by BG + GEN (4.2 Ncm), BG (3.3 Ncm), and CLOT (2.1 Ncm). BG + GEN and BO + GEN upregulated the bone markers (RANKL, OPG, OCN, IBSP) (p < 0.05). Micro-CT revealed higher bone volume percentage (BV/TV) in BO + GEN (11.67%) and BG + GEN (10.51%) versus their controls (p < 0.05). Trabecular separation (Tb.Sp) was significantly reduced in BO + GEN compared to BG (p < 0.05). These findings suggest that genistein can potentiate the osteoconductive properties of clinically used biomaterials, enhancing peri-implant bone repair in ovariectomized rats. ## Linked entities - **Genes:** TNFSF11 (TNF superfamily member 11) [NCBI Gene 8600], BTF3P11 (basic transcription factor 3 pseudogene 11) [NCBI Gene 690], BGLAP (bone gamma-carboxyglutamate protein) [NCBI Gene 632], IBSP (integrin binding sialoprotein) [NCBI Gene 3381] - **Chemicals:** genistein (PubChem CID 5280961) - **Diseases:** osteoporosis (MONDO:0005298) - **Species:** Rattus norvegicus (taxon 10116) ## Full-text entities - **Genes:** Ctnnb1 (catenin beta 1) [NCBI Gene 84353] {aka Catnb}, Wnt2 (Wnt family member 2) [NCBI Gene 114487] {aka Wnt}, Esr1 (estrogen receptor 1) [NCBI Gene 24890] {aka ER-alpha, Esr, RNESTROR}, Tnfrsf11b (TNF receptor superfamily member 11B) [NCBI Gene 25341] {aka Opg}, Ibsp (integrin-binding sialoprotein) [NCBI Gene 24477] {aka Bsp}, Bglap (bone gamma-carboxyglutamate protein) [NCBI Gene 25295] {aka Bglap2, Bgp, Bgpr, Bgpra}, Tnfsf11 (TNF superfamily member 11) [NCBI Gene 117516] {aka ODF, OPGL, RANKL, TRANCE}, Actb (actin, beta) [NCBI Gene 81822] {aka Actx}, Vegfa (vascular endothelial growth factor A) [NCBI Gene 83785] {aka VEGF-A, VEGF111, VEGF164, VPF, Vegf} - **Diseases:** Menopause (MESH:D008594), overdose (MESH:D062787), analgesia (MESH:D000699), Estrogen (MESH:D056828), peri-implant (MESH:D057873), toxicity (MESH:D064420), BO (MESH:D001847), osteoporotic (MESH:D058866), ridge deficiencies (MESH:C565110), atrophic (MESH:D020966), osteoporosis (MESH:D010024), skeletal disease (MESH:D004194), fractures (MESH:D050723), spongiform encephalopathy (MESH:D016098) - **Chemicals:** Polyglactin 910 (MESH:D011098), hydrogen (MESH:D006859), alcohol (MESH:D000438), tramadol (MESH:D014147), Bio-Oss (MESH:C077540), risedronate (MESH:D000068296), penicillin G-benzathine (MESH:D010401), PVPI (MESH:D011206), formaldehyde (MESH:D005557), calcium (MESH:D002118), CaO (MESH:C016538), water (MESH:D014867), sodium oxide (MESH:C096707), benzoyl peroxide (MESH:D001585), ketamine hydrochloride (MESH:D007649), Calcein (MESH:C007740), Trizol (MESH:C411644), C15H10O5 (MESH:D019833), 17beta-estradiol (MESH:D004958), P2O5 (MESH:C012500), isoflavone (MESH:D007529), nitrogen (MESH:D009584), titanium (MESH:D014025), xylazine hydrochloride (MESH:D014991), hydroxyapatite (MESH:D017886), acetone (MESH:D000096), methyl methacrylate (MESH:D020366), BioRender (-), D (MESH:D003903), alizarin (MESH:C010078), sodium chloride (MESH:D012965), lidocaine (MESH:D008012), phosphate (MESH:D010710), SiO2 (MESH:D012822), sodium thiopental (MESH:D013874), sulfuric acids (MESH:D013464), sodium (MESH:D012964) - **Species:** Bos taurus (bovine, species) [taxon 9913], Rattus norvegicus (brown rat, species) [taxon 10116], Homo sapiens (human, species) [taxon 9606] ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12913274/full.md ## Figures 6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12913274/full.md ## References 1 references — full list in the complete paper: https://tomesphere.com/paper/PMC12913274/full.md --- Source: https://tomesphere.com/paper/PMC12913274