# Investigation of attachment and proliferation of MG-63 osteoblast-like cells to titanium disks in the presence of iPRF (injectable platelet-rich fibrin) or CGF (Concentrated Growth Factor): A laboratory study

## TL;DR

This study compares how iPRF and CGF affect the attachment and growth of bone cells on titanium surfaces, finding that CGF has a stronger short-term effect.

## Contribution

The novel contribution is the comparison of short-term and long-term effects of iPRF and CGF on MG-63 cell behavior on titanium surfaces.

## Key findings

- CGF showed better cell attachment than iPRF in the first 24 hours.
- No significant differences were observed between iPRF, CGF, and the control group after 48 hours.
- The initial superiority of CGF in promoting cell survival and proliferation diminished over time.

## Abstract

The attachment of the dental implant to the host bone (osteointegration) is considered a critical factor in the success of implant treatment. Osteoblasts are the primary cells in the osteointegration process. Today, compounds containing growth factors are used to shorten osteointegration time and increase the treatment success rate. This study evaluated the effects of iPRF (injectable platelet-rich fibrin) and CGF (concentrated growth factor) platelet extracts on the attachment and proliferation of MG-63 osteoblast-like cells over titanium disk surfaces.

Titanium pieces with a length of 12.1 mm and a width of 3.8 mm were prepared. The MG-63 cell viability, attachment, and proliferation on titanium disks were evaluated in the presence of prepared extracts from iPRF and CGF using methyl thiazolyl tetrazolium (MTT) assay and scanning electron microscopy (SEM).

The results of the direct exposure of different concentrations of extracts on MG-63 cells cultured on the polystyrene surface of cell culture plates in the absence of fetal bovine serum (FBS) showed no significant difference between different concentrations of iPRF and CGF extracts in the first 24 hours after exposure (P>0.05). However, 48 hours after exposure, CGF extracts showed better effects (P<0.05). In the first 24 hours, MG-63 cell attachment to the titanium disks was significantly higher after exposure to CGF compared to iPRF and the control group (P<0.05). Nevertheless, 48 hours after culturing, no significant differences were observed in MG-63 survival, proliferation, and attachment between iPRF, CGF, and the control group (absence of iPRF and CGF) (P>0.05).

The results showed a better short-term (first 24 hours after exposure) effect of CGF on primary cell survival, attachment, and proliferation compared to iPRF; however, this superiority disappeared over time.