# Exosome-Derived circ0009910 Promotes Pituitary Adenoma Cell Proliferation, Invasion, Migration, and EMT through the miR-106b-5p/STAT3 Axis

## TL;DR

This study shows that exosome-derived circ0009910 promotes pituitary adenoma growth and spread by interacting with miR-106b-5p and STAT3.

## Contribution

The study identifies a novel regulatory mechanism involving exosome-derived circ0009910 in pituitary adenoma progression.

## Key findings

- circ0009910 is transferred via exosomes and promotes PA cell proliferation and migration.
- miR-106b-5p acts as a sponge for circ0009910 and reverses its effects.
- circ0009910 knockdown inhibits tumor growth and EMT in vivo.

## Abstract

This study aimed to explore whether exosome-derived circ0009910 can transcellularly regulate the growth of pituitary adenoma (PA) cells and to further explore the possible mechanisms of its action.

Transmission electron microscopy and nanoparticle size analysis were used to observe the morphology and size of the exosomes. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to determine the expression of circ_0009910, miR-106b-5p, and signal transducer and activator of transcription3 (STAT3). Western blotting was used to assess the expression of exosomal marker proteins, p-STAT3, E-cadherin, N-cadherin, and vimentin. Cell Counting Kit-8 (CCK-8) and 5-Ethynyl-2-deoxyuridine (EdU) assays were used to determine the proliferative capacity of the cells. Transwell assays were performed to assess the migratory and invasive capacity of the cells. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the expression level of growth hormone (GH). A nude mouse xenograft model was established to observe the effects of exosome-derived circ0009910 on transplanted tumors in nude mice.

circ0009910 can be transferred to other cells via exosomes. Knocking down the expression of circ0009910 can inhibit the proliferation, invasion, and migration of PA cells, reduce GH expression, and regulate the expression of epithelial–mesenchymal transition (EMT)-associated proteins. miR-106b-5p is a molecular sponge of circ0009910 and can partially reverse the procarcinogenic effect of circ0009910 in PA.
STAT3
is a target gene of miR-106b-5p. In addition, circ0009910 knockdown inhibited tumor growth in vivo.

Exosome-derived circ0009910 promotes PA progression and regulates EMT through the miR-106b-5p/STAT3 axis.