Macrophage‐Mediated Cellular Communication Networks in Lung Squamous Cell Carcinoma and Adenocarcinoma Revealed by Single‐Cell Sequencing
Xiaoyu Zhang, Yunlong Zhao, Yingying Wang, Xiaomin Yu, Hongyu Xia, Meiru Li, Xiu-An Yang
TL;DR
This study uses single-cell sequencing to uncover how macrophages interact with cancer cells in two types of lung cancer, revealing potential targets for personalized treatments.
Contribution
The study identifies subtype-specific epithelial signatures and macrophage-mediated signaling pathways in LUSC and LUAD.
Findings
Four epithelial signatures were identified, with S3 specific to LUSC and linked to high malignancy.
Macrophages interact with cancer cells via SPP1-CD44 in LUSC and RESISTIN-CAP1 in LUAD.
CD44 and CD74 expression correlates with prognosis in LUSC and LUAD, respectively.
Abstract
Lung cancer, particularly the non‐small cell lung cancer (NSCLC) subtypes lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD), exhibits high heterogeneity and high mortality. This study aimed to explore their tumor microenvironment (TME) features, cellular interactions, and potential therapeutic targets. Using scRNA‐seq datasets (GSE200972, GSE117570, and GSE127465) and TCGA bulk RNA‐seq data, we performed cell clustering, pseudotime trajectory, cell–cell communication, and survival analyses. Batch correction and quality control were applied first, followed by cell type annotation with SingleR, copy number variation inference with InferCNV, and intercellular signaling investigation with CellChat. Four epithelial signatures (S1–S4) with distinct gene expression profiles were identified, with S3 specific to LUSC and correlated with high malignancy. Pseudotime analysis…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17
Figure 18
Figure 19
Figure 20
Figure 21
Figure 22
Figure 23
Figure 24
Figure 25
Figure 26
Figure 27
Figure 28
Figure 29
Figure 30
Figure 31
Figure 32
Figure 33
Figure 34
Figure 35
Figure 36
Figure 37
Figure 38
Figure 39
Figure 40
Figure 41
Figure 42
Figure 43
Figure 44
Figure 45
Figure 46
Figure 47
Figure 48
Figure 49
Figure 50Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsSingle-cell and spatial transcriptomics · GDF15 and Related Biomarkers · Ferroptosis and cancer prognosis