Biomimetic hydrogel for the construction of patient-derived bladder cancer organoids with aggressive growth
Jin Zhang, Jiaxin Wang, Xiaofeng Hu, Wei Jia, Ziyuan Zhou, Gaohaer Kadeerhan, Wenmin Guo, Jun Tian, Hong Guo, Ling Guo, Dongwen Wang
TL;DR
This study creates bladder cancer organoids using a biomimetic hydrogel that better mimics tumor behavior and drug responses for personalized treatment.
Contribution
A novel hydrogel using cellulose microfibers to mimic tumor collagen improves bladder cancer organoid growth and drug sensitivity.
Findings
Cellulose microfibers enhance organoid viability, invasiveness, and migration.
Organoids preserved tumor architecture and mutations, showing drug sensitivities similar to patient tissues.
The model correlates with patient outcomes, suggesting utility for preclinical therapeutic testing.
Abstract
In cancer, the extracellular matrix (ECM) reorganization, notably collagen, forming dense, thickened, and orderly structures, affects tumor traits. Bladder cancer organoids (BCOs) mimic tumor properties for personalized medicine. However, current organoid scaffolds lack tumor-such as collagen, crucial for cell growth and migration. Previous efforts to incorporate mesoscale collagen fibers extracted directly from tumors into scaffolds were limited by the size of the tumor tissue and the efficiency of extraction. In this study, we used cellulose microfibers (MCFs) to mimic in vivo mesoscale collagen’s role, enhancing BCO viability, invasiveness, and migration, aligning with tumor growth patterns. These organoids preserved tumor architecture and mutations, showing drug sensitivities similar to those of parental tissue-derived cells and correlating with patient outcomes. This suggests that…
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Taxonomy
TopicsCancer Cells and Metastasis · Tissue Engineering and Regenerative Medicine · Proteoglycans and glycosaminoglycans research