Gene mapping and development of molecular markers for thousand-grain weight in rye based on bulked segregant analysis
Lai Wei, Zhenbo Zhai, Yunjie Yang, Yanping Yang, Yonghe Che
TL;DR
This study identifies genes and molecular markers linked to grain weight in rye, using a wild and cultivated rye cross to improve breeding efforts.
Contribution
The study introduces new molecular markers and a gene linked to thousand-grain weight in rye using BSA-seq.
Findings
BSA-seq identified 10 genomic regions with 68 SNPs associated with thousand-grain weight in rye.
Six KASP and one SSR marker were developed, with TGW-16 proving effective for selecting high-yield germplasm.
A candidate gene encoding an RNA recognition motif protein was linked to TGW.
Abstract
Xinjiang wild rye (Secale cereale subsp. segetale) is a wild rye species that was discovered in Xinjiang Province of China in the 20th century. It is a relatively understudied cereal crop within the Poaceae family, and there is currently a lack of research on its yield-related traits. Mapping genes controlling thousand-grain weight (TGW) is crucial for developing high-yielding rye cultivars. In this study, bulk segregant analysis sequencing (BSA-seq) was performed on a cross-pollinated (CP) hybrid population derived from cultivated rye (Z837) and Xinjiang wild rye (90R13) to identify genomic regions associated with TGW. Molecular markers were then developed in the region of the initially mapped genes to further localize the TGW gene. BSA-seq analysis identified 10 candidate regions containing 68 single nucleotide polymorphism (SNP) loci across the seven chromosomes and unassembled…
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Taxonomy
TopicsWheat and Barley Genetics and Pathology · Genetic Mapping and Diversity in Plants and Animals · Plant and fungal interactions