# Protocol for the synthesis of N-Alkyl bromomaleimide linkers

## TL;DR

The paper presents a reliable method to synthesize bromomaleimide linkers with different chain lengths for use in molecular modification.

## Contribution

A new synthetic protocol for bromomaleimides with varied chain lengths and bifunctional potential is introduced.

## Key findings

- The method produces high-purity bromomaleimides in good to excellent yields.
- Compound 5's bifunctional structure allows for dual payload conjugation.
- Bromomaleimides show greater stability and selectivity for Cysteine modification compared to maleimides.

## Abstract

Herein a straightforward and efficient method for synthesizing bromomaleimides with varied carbon chain lengths to modulate molecular properties has been reported. Compounds 1–4 incorporate a single bromomaleimide unit, while compound 5 features two, enabling dual functionalization. This synthetic approach consistently yields high-purity products in good to excellent yields, with compound 5 offering potential for dual payload conjugation due to its bifunctional structure. The key highlights are:

1. Bromomaleimides offers exceptional stability compared to maleimides counterparts

2. Highly selective for Cysteine modification

Image, graphical abstract