# New aspirin-chitosan conjugates as potential anti-Staphylococcus aureus agents

## TL;DR

Researchers created new aspirin-chitosan compounds that show strong antibacterial effects against drug-resistant Staphylococcus aureus.

## Contribution

The paper introduces two novel aspirin-chitosan conjugates with enhanced antibacterial activity against MRSA.

## Key findings

- The Cs/3a conjugate significantly reduced S. aureus growth compared to other formulations.
- Amide linkages were successfully formed between chitosan and aspirin derivatives.
- Characterization techniques confirmed the structural properties of the conjugates.

## Abstract

Two novel aspirin-chitosan conjugates were successfully designed and synthesized: A phenyl acetate derivative (PAD)/chitosan conjugate and an acetoxybenzoate derivative (ABD)/chitosan conjugate. The conjugates were prepared via freeze-drying methodology, where chitosan was reacted with the phenyl acetate derivative (PAD) 3a and the acetoxybenzoate derivative (ABD) 3b through nucleophilic attack of chitosan’s amino groups on the carbonyl carbons of the aspirin-containing derivatives, forming stable amide linkages. The resulting conjugates were comprehensively characterized using infrared spectroscopy (IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) to confirm successful conjugation and evaluate structural properties. Antibacterial activity was assessed against methicillin-resistant Staphylococcus aureus (MRSA) using colony-forming unit (CFU) assays. Results demonstrated that the Cs/3a conjugate exhibited superior antibacterial efficacy, achieving a significant reduction in S. aureus growth compared to other formulations. These findings suggest that aspirin-chitosan conjugation represents a promising strategy for developing antimicrobial biomaterials with enhanced therapeutic potential against drug-resistant bacterial pathogens.

The online version contains supplementary material available at 10.1186/s13065-025-01712-x.