# Exploring the bioactive potential of bovine pericardium membrane combined with hyaluronic Acid: characterization and cellular viability analyses

## TL;DR

This study shows that adding hyaluronic acid to bovine pericardium membranes improves their properties and supports cell growth, making them suitable for tissue engineering.

## Contribution

The novel contribution is demonstrating the bioactive potential of hyaluronic acid-modified bovine pericardium membranes for tissue engineering.

## Key findings

- Hyaluronic acid modification increased membrane hydrophilicity and altered physicochemical characteristics.
- Fibroblast cell viability was maintained without cytotoxic effects on HA-modified membranes.
- FTIR and SEM confirmed favorable structural and morphological properties for cell-material interactions.

## Abstract

This study explores the bioactive potential of bovine pericardium membranes combined with hyaluronic acid (HA, 120 kDa) at concentrations of .5%, 1.0%, and 2.0% through physicochemical characterization and fibroblast cell viability analysis. A laboratory experimental design was employed to evaluate bovine pericardium membranes modified with hyaluronic acid at different concentrations. Material characterization was conducted employing Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analyses. Fibroblast cell viability was assessed using the MTT assay, and statistical analysis was conducted using the ANOVA test. The results demonstrated that hyaluronic acid modification altered the physicochemical characteristics of the membrane, including increased surface hydrophilicity. The cell viability test revealed comparable fibroblast viability among HA-modified membranes, indicating no cytotoxic effects. FTIR and SEM analyses confirmed chemical interactions and morphological features associated with favorable cell–material interactions. These findings suggest that bovine pericardium membrane with hyaluronic acid 120 kDa exhibit favorable physicochemical characteristics and in vitro cytocompatibility, indicating their potential for tissue engineering applications. This research provides a scientific foundation for developing innovative biomaterials that support favorable cell–material interactions for tissue regeneration research, contributing to advancements in tissue regeneration therapy.

•Bovine pericardium membranes were modified with hyaluronic acid (120 kDa).•Hyaluronic acid incorporation altered membrane physicochemical characteristics.•FTIR and SEM analyses confirmed preserved collagen structure and surface morphology.•HA-modified membranes showed comparable fibroblast viability with no cytotoxic effects.•The membranes demonstrate potential as biocompatible biomaterials for tissue engineering applications.

Bovine pericardium membranes were modified with hyaluronic acid (120 kDa).

Hyaluronic acid incorporation altered membrane physicochemical characteristics.

FTIR and SEM analyses confirmed preserved collagen structure and surface morphology.

HA-modified membranes showed comparable fibroblast viability with no cytotoxic effects.

The membranes demonstrate potential as biocompatible biomaterials for tissue engineering applications.