Accurate monitoring of substrate-dependent growth reveals ecotypic differentiation among marine yeasts

TL;DR

This study shows how marine yeasts break down phytoplankton-derived sugars and contribute to carbon cycling in the ocean.

Contribution

A novel protocol was developed to study marine yeast growth on specific substrates with high temporal resolution.

Findings

Four distinct yeast ecotypes were identified based on their substrate utilization patterns.

Yeast degradation of laminarin produces diverse short-chained intermediates, increasing chemical complexity.

Yeast ecotypes match abundant OTUs in marine mycoplankton datasets, indicating their ecological importance.

Abstract

Phytoplankton-derived dissolved organic matter (DOM) sustains complex marine microbial communities, yet the role of marine fungi—particularly yeasts—remains understudied regarding their substrate preferences, enzymatic strategies, and ecological relevance. We developed a novel protocol to investigate substrate-specific growth of marine fungal isolates under defined conditions and high temporal resolution. Using the β-1,3-glucan laminarin—a major marine storage polysaccharide of phytoplankton—and its oligomeric and monomeric breakdown products, we characterized growth and substrate utilization profiles of eleven marine yeast isolates from the epipelagic zone at Helgoland Roads, North Sea. Statistical analyses of growth kinetics distinguished four ecotypes with distinct substrate utilization patterns, quantified via phenol–sulfuric acid assays. Fluorophore-assisted carbohydrate electrophoresis (FACE) revealed the lack of endo-laminarinase activity, suggesting laminarin degradation depends on exo-acting enzymes. FACE also revealed a high diversity of short-chained laminarin-based intermediates accumulating over time, demonstrating that yeasts enhance chemical complexity during laminarin degradation and may fuel other microbes within the microbial loop. Representatives of each yeast ecotype were found to match abundant operational taxonomic units (OTU) in sequence similarity analyses of epipelagic mycoplankton datasets. This supports their ecological success and diverse substrate strategies. Rather than acting solely as opportunists, these yeasts may actively shape DOM turnover and carbon cycling within the microbial loop. Our study highlights a robust experimental approach for resolving functional diversity among marine yeasts and underpins their potential role in maintaining chemical diversity and substrate cross-feeding in the microbial loop.

Graphical Abstract

Copyright: OpenStreetMap contributors. Data licensed under the Open dAtabase Lecense (ODbL) v1.0.

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