Baicalin as a Regulator of Peroxisome Proliferator‐Activated Receptor Gamma: Alleviating Sepsis‐Induced Liver Injury by Inhibiting the Cluster of Differentiation 14/Nuclear Factor Kappa B Signaling Pathway

TL;DR

Baicalin helps reduce liver damage from sepsis by activating PPARγ and blocking the CD14/NF-κB pathway.

Contribution

This study reveals a novel mechanism of baicalin in alleviating sepsis-induced liver injury via PPARγ and CD14/NF-κB.

Findings

Baicalin improved cell viability and reduced apoptosis in LPS-treated AML12 cells.

Baicalin downregulated pro-inflammatory cytokines and inhibited the CD14/NF-κB pathway.

PPARγ silencing reversed the protective effects of baicalin, confirming its role in the mechanism.

Abstract

Sepsis frequently results in multiple organ failure, with the liver particularly susceptible to sepsis‐induced damage. The flavonoid baicalin (BA), derived from Scutellaria baicalensis, presents anti‐inflammatory properties, yet its effects on liver injury in sepsis through the Cluster of Differentiation 14 (CD14)/Nuclear Factor kappa B (NF‐κB) pathway remain underexplored.

Alpha Mouse Liver 12 (AML12) cells were intervened with different concentrations of BA and subsequently challenged with lipopolysaccharide (LPS) to induce inflammation. Cell viability, apoptosis, inflammatory cytokine production, and signaling pathway activation were tested by Cell Counting Kit 8 (CCK8), flow cytometry, quantitative real‐time PCR (qRT‐PCR) and western blotting, respectively. And immunofluorescence was employed to visualize nuclear ectopic location of NF‐κB in cells. Additionally, the impact of peroxisome proliferator‐activated receptor gamma (PPARγ) silencing for BA's function was explored using small interfering RNA (siRNA) techniques.

BA significantly improved viability and decreased apoptosis in LPS‐treated cells. It also downregulated tumor necrosis factor alpha (TNF‐α), interleukin‐1 beta (IL‐1β) and IL‐6, upregulated PPARγ, and diminished CD14 and phosphorylated inhibitor of kappa B alpha (p‐IKBα)/IKBα levels. Silencing PPARγ reversed the protective effects of BA, underscoring the importance of PPARγ in BA's mechanism of action.

BA alleviates sepsis‐induced liver injury through activating PPARγ and inhibiting CD14/NF‐κB pathway, highlighting its potential as a new approach to treatment sepsis.

Baicalin (BA) alleviates sepsis‐induced liver injury through activating peroxisome proliferator‐activated receptor gamma (PPARγ) and inhibiting the Cluster of Differentiation 14 (CD14)/Nuclear Factor kappa B (NF‐κB) pathway.

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