Evaluation of the Osteogenic Potential of Apigenin via Inducing Autophagy: An In Vitro Study
Mozafar Khazaei, Amirmohammad Khodaei, Maryam Bozorgi, Mohammad Rasool Khazaei, Azam Bozorgi
TL;DR
This study shows that apigenin boosts bone cell development in stem cells by triggering autophagy, a process that helps cells renew.
Contribution
The novel finding is that apigenin enhances osteogenic differentiation of ASCs through autophagy induction.
Findings
Apigenin treatment increased expression of osteogenic and autophagy-related genes and proteins in ASCs.
Alkaline phosphatase activity and calcium deposition were significantly higher in apigenin-treated groups.
Apigenin promotes osteogenic differentiation of ASCs via autophagy stimulation.
Abstract
The present study aimed to evaluate the effect of apigenin (Api) on the osteogenic differentiation of adipose tissue‐derived mesenchymal stem cells (ASCs) by stimulating autophagy. ASCs were isolated from fresh adipose tissues using mechanical and enzymatic digestion and characterized using flow cytometry. ASCs were treated with Api (0, 5, 10, 25, and 50 µM) for 48 and 72 h. After determining the optimal doses of Api (5 and 10 µM), ASCs were differentiated into the osteogenic lineage for 7 and 21 days. The expression of osteogenic and autophagy genes and proteins, as well as alkaline phosphatase (ALP) activity and calcium deposition, were assessed. About 94% of ASCs expressed CD73, CD90, and CD105, while 99% didn’t express CD34 and CD45. Api treatment increased the expression of ALP, RUNX2, COL I, osteocalcin (OCN), ATG5, ATG7, and LC3A genes, and RUNX2, OCN, LC3‐1, and LC3‐II proteins…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17
Figure 18Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsAutophagy in Disease and Therapy · Bone Metabolism and Diseases · Mesenchymal stem cell research