# Poster Session II - A202 COMPARISON OF INVASIVE HELICOBACTER PYLORI DETECTION METHODS IN PATIENTS UNDERGOING GASTROSCOPY IN A CANADIAN TERTIARY CARE CENTER

## TL;DR

This study compares three invasive methods for detecting Helicobacter pylori in patients and finds histopathology to be the most accurate.

## Contribution

The study evaluates the accuracy of histopathology, RUT, and culture for H. pylori detection in a Canadian setting.

## Key findings

- Histopathology had the highest accuracy for detecting H. pylori compared to RUT and culture.
- Only 3.3% of cases were positive by RUT, the lowest among the three methods.
- Age over 50 years was the only predictor of H. pylori prevalence and histopathology positivity.

## Abstract

Helicobacter pylori (HP) is a prevalent gastrointestinal bacterium that predisposes individuals to a variety of gastrointestinal disorders. Various invasive testing methods are available including culture, rapid urease testing (RUT) and histopathology, however no gold standard exists and there is heterogeneity in the accuracy of these across various studies.

To compare the accuracy of three invasive diagnostic techniques (histopathology, RUT and culture) for HP and determine predictors of positive results for each. Our secondary aim is to characterize the prevalence of HP infection in Manitoba

Retrospective single center study of adult patients undergoing gastroscopy and biopsy for HP testing. For each patient, biopsies were taken for histopathological, RUT and culture. Patient demographics including age, sex, and medication use (PPI, H2RA, antibiotics and anticoagulants/NSAIDs) were collected. Procedure related data collected included indication for gastroscopy, endoscopic findings and biopsy results. The presence of HP and active or inactive gastritis was reported. Regression analysis was performed to determine predictors of positive findings on histopathology, RUT, culture and HP prevalence.

Of the 142 cases analyzed there were 30 positive H. pylori results. 29 (96.6%) were positive by histopathology, 17(56.6%) were positive by culture, and only 1(3.3%) was positive by RUT. One case that was not positive by histopathology was positive by culture. All positive cases were accompanied by either active or inactive gastritis. After multivariate regression analysis, only age >50 years was predictive of histopathology (OR 1.17, 95% CI 1.01,1.34; p = 0.036) and prevalence (OR 1.17, 95% CI 1.02,1.36; p = 0.028). The prevalence of HP in our study was 21.1%. Using histopathology as the gold standard, we report a culture sensitivity of 58.6% and specificity of 92.3%.

Histopathology shows the highest accuracy for diagnosing HP in our single center study, despite previous literature suggesting RUT as a highly sensitive and specific test for H. pylori detection. RUT had the lowest accuracy of the three tests we assessed which may be due to site-specific factors and should therefore be used with caution. Age was the only predictive variable of HP prevalence and histopathologic positivity.

St. Boniface Research Center, Winnipeg, MB.