# Mentha piperita Essential Oil in Olive Oil: Extending Erythrocyte Viability and Limiting Bacterial Growth Under Serum-Free Conditions

## TL;DR

Mentha piperita essential oil in olive oil helps red blood cells survive longer and reduces bacterial growth in serum-free conditions.

## Contribution

ATP-oil® extends erythrocyte viability and limits bacterial contamination in serum-free cultures.

## Key findings

- Genuine ATP-oil® maintained 35–45% RBC viability at day 18, outperforming controls.
- ATP-oil® showed a qualitative reduction in bacterial contamination compared to controls.
- Mimicking preparations did not replicate the effects of genuine ATP-oil®.

## Abstract

Background: Serum-free culture of red blood cells (RBCs) typically leads to rapid loss of viability, limiting experimental and translational applications. Lipid-rich formulations and essential oils may provide biocompatible support for RBC integrity while limiting microbial overgrowth. Methods: RBCs from nine healthy adult donors were cultured in serum-free RPMI under four conditions: control, vehicle (olive oil, 1:100 v/v), genuine adenosine triphosphate (ATP)-oil® (1:100 v/v), and laboratory oil, “mimicking” ATP-oil®. Cultures were maintained for 18 days. Viability was assessed by light microscopy and trypan blue exclusion; bacterial contamination was qualitatively observed on day 18. Results: Genuine ATP-oil® maintained 35–45% RBC viability at day 18, whereas control and vehicle cultures declined rapidly. The mimicking preparation did not reproduce these effects. ATP-oil® immersion was associated with a qualitative reduction in bacterial contamination versus control, consistent with a dual action on RBC preservation and microbial suppression under serum-free conditions. Conclusions: Supplementation with ATP-oil® substantially prolongs RBC survival and limits bacterial overgrowth in vitro, outperforming commonly used serum or plasma supplements on a per-volume basis. These findings suggest potential applications for improving ex vivo handling or storage of blood components and for reducing background contamination in diagnostic microbiology. Further studies with larger cohorts are warranted to reveal underlying mechanisms and to define active constituents in order to standardize production.