# Development of a High-Throughput UHPLC-DMS-MS/MS Method for Targeted Quantitation of Pertinent Phospholipid Classes in Colon Cancer

## TL;DR

A new method for measuring specific phospholipids in colon cancer tissue was developed, enabling precise quantification and revealing significant differences between tumor and non-tumor tissues.

## Contribution

A novel LC–DMS–MS/MS method was developed for absolute quantification of phospholipids with high selectivity and sensitivity.

## Key findings

- The method quantified 47 phospholipid species in colon cancer tissues with low ng per mg tissue limits of quantification.
- 22 phospholipid species were significantly increased in tumor tissue compared to non-tumor tissue.
- Phosphatidylglycerol and phosphatidylethanolamine species showed the largest fold changes in tumor tissues.

## Abstract

Phospholipids are essential membrane constituents that regulate diverse cellular processes, yet most current workflows rely on relative quantification using high-resolution LC–MS. We developed and validated a highly selective targeted method that couples liquid chromatography with differential mobility spectrometry and tandem mass spectrometry (LC–DMS–MS/MS), providing enhanced selectivity and reduced background noise. The assay quantifies 63 phospholipid species across four classes, achieving excellent recoveries and limits of quantification in the low ng per mg tissue range. Applied to tissues from a colon cancer study in mice, the method enabled the absolute quantification of 47 species, 22 of which were significantly increased in tumor tissue versus adjacent non-tumor tissue. While phosphatidylcholines were the most abundant class overall, the largest fold changes were observed in long-chain phosphatidylglycerol and phosphatidylethanolamine species. LC–DMS–MS/MS thus offers a robust, selective platform for absolute phospholipid quantification and for detecting disease-associated lipid remodeling.