# Design and Application of Fluorescence Probes for Gold Nanocage Complex Perovskite Quantum Dots

## TL;DR

This study designs a fluorescent probe using gold nanocages and perovskite quantum dots for detecting lung cancer tumor markers with high sensitivity.

## Contribution

A novel composite fluorescent probe combining gold nanocages and perovskite quantum dots for tumor marker detection is developed.

## Key findings

- The fluorescence intensity of the composite system was enhanced by 15.38% compared to pure perovskite quantum dots.
- The probe effectively detects miRNA-4529-3P and miR-301b-3p, tumor markers for non-small cell lung cancer.
- The probe demonstrates excellent recognition performance through FRET disruption upon target hybridization.

## Abstract

In this study, a gold nanocage composite perovskite quantum dot fluorescent probe (MB-GNCs-PQDs) was designed and constructed. The GNCs-PQDs composite system was formed by the combination of gold nanocages (GNCs) and perovskite quantum dots (PQDs). Spectral analysis confirmed that its fluorescence intensity was significantly enhanced by 15.38% compared with that of pure PQDs. Furthermore, amino modification was performed on the nanomaterial. Through the specific design of molecular beacons (MB), the fluorescence emission spectrum of the probe was matched with the absorption peak of the quencher group BHQ2, and the effective closure of the fluorescence signal was achieved based on the Fluorescence Resonance Energy Transfer (FRET) effect. Subsequently, MB was immobilized on the surface of the composite system via amino covalent conjugation to complete the probe preparation. The prepared probe was applied to the detection of miRNA-4529-3P and miR-301b-3p, which are tumor markers of non-small cell lung cancer (NSCLC). The hybridization of target molecules with MB could trigger the disruption of FRET and the recovery of fluorescence signal, exhibiting excellent recognition performance. This study provides an experimental basis for the preparation of composite fluorescent probes, and the developed probe has potential application value in the field of tumor marker detection.