# Essential Role of LapD in the Absence of Cardiolipins

## TL;DR

This study explores how the absence of cardiolipins in E. coli affects bacterial survival and reveals the regulatory role of LapD in lipid synthesis.

## Contribution

The study identifies LapD as a key regulator linking LPS assembly and fatty acid/phospholipid synthesis in E. coli.

## Key findings

- CL synthesis is conditionally essential in ΔlapD bacteria.
- Loss-of-function mutations in cdsA and pgsA bypass lethality by reducing acidic phospholipids.
- Overexpression of accC and glnB overcomes Δ(lapD clsA) lethality by inhibiting fatty acid/PL synthesis.

## Abstract

To maintain the integrity of the outer membrane of Gram-negative bacteria, such as Escherichia coli, the levels of two essential components, phospholipids (PL) and lipopolysaccharide (LPS), are tightly regulated, although the underlying molecular mechanisms are unclear. E. coli synthesizes three main PLs, including essential phosphatidylethanolamine and phosphatidylglycerol and nonessential cardiolipin (CL). We showed that CL synthesis is conditionally essential in ΔlapD bacteria. Using this synthetic lethal phenotype, we isolated suppressors that rescued growth at elevated temperatures. We showed that loss-of-function mutations in cdsA encoding CDP-diglyceride synthetase, and pgsA, which encodes phosphatidylglycerophosphate synthase, bypass this lethality. Such mutations reduce the relative abundance of acidic phospholipids, which are otherwise elevated in Δ(lapD clsA) bacteria, and increase the amounts of cis-vaccenic acid without altering amounts of LpxC mediating the first committed step in LPS biosynthesis. Interestingly, overexpression of genes, including accC and glnB, whose products can inhibit fatty acid/PL synthesis, overcame the lethality of Δ(lapD clsA) bacteria. We demonstrated that PgsA co-purifies with LapB, which regulates LpxC stability and acts as a hub for proteins involved in PL and LPS biosynthesis, including LapD. Overall, our results reveal that LapD is positioned at the regulatory nexus between LPS assembly and fatty acid/PL synthesis.