# Synthesis of New Volatile Derivatives of Biogenic Amines, Carbamates for Analytical Applications

## TL;DR

This paper introduces a new method to create volatile carbamate derivatives of biogenic amines for better analytical performance in food testing.

## Contribution

A new derivatization strategy using TFECF for biogenic amines with improved volatility and analytical performance is presented.

## Key findings

- TFECF derivatives were obtained in high yields (94–99%) and confirmed with NMR, MS, and crystal analysis.
- TFECF derivatives showed improved volatility and shorter retention times compared to ECF derivatives in GC-MS.
- The method was successfully applied to real food matrices, demonstrating its practical utility.

## Abstract

In this study, a comprehensive derivatization strategy for biogenic amines based on the formation of volatile carbamate derivatives using 2,2,2-trifluoroethyl chloroformate (TFECF) was successfully developed and evaluated. A series of biogenic amine derivatives was obtained in excellent yields (94–99%) and structurally confirmed using NMR, MS, and crystal structure analysis. The reagent demonstrated high reactivity toward primary and secondary amines, providing derivatives of excellent purity and satisfactory volatility. The applicability of the proposed procedure to real food matrices was demonstrated using GC-MS. The obtained results were compared with the corresponding ethyl chloroformate (ECF) derivatives. TFECF derivatives exhibited significantly improved volatility, reflected in shorter retention times and enhanced analytical performance.