# Functional Characterization of Eccyp307a1 in Early Ovary Development of Exopalaemon carinicauda

## TL;DR

This study investigates the role of Eccyp307a1 in the early development of ovaries in the crustacean Exopalaemon carinicauda.

## Contribution

The study reveals the functional role of Eccyp307a1 in crustacean ovary development, which was previously unknown.

## Key findings

- Eccyp307a1 is highly expressed during early ovary development in E. carinicauda.
- Eccyp307a1 is regulated by neuroendocrine factors and is essential for normal ovary development initiation.
- The Eccyp307a1 protein contains typical P450 domains and heme-binding sites.

## Abstract

According to a previous study, in insects, cyp307A1 plays a central role in ecdysteroid synthesis, which is a member of the cytochrome P450 family. However, the function of cyp307A1 in crustaceans remains unclear. In this study, we explored the function of Eccyp307a1 in Exopalaemon carinicauda through a series of experiments. The sequence of Eccyp307a1 encoded 529 amino acids, and the protein was found to possess typical P450 domains and heme-binding sites. The mRNA of Eccyp307a1 was expressed at a higher level during the early stages of ovary development, but was expressed less during the mature stage. Furthermore, employing eyestalk ablation and RNAi experiments, we determined that Eccyp307a1 could be regulated by neuroendocrine factors and is essential for the normal initiation of ovary development. These findings provided insights into the gene function of Eccyp307a1 in early ovary development in E. carinicauda, and our study further elucidates the molecular mechanisms of ovary development in crustaceans.