# Chloride-Transporting OsHKT1;1 Splice Variants and Their Expression Profiles Under Salinity Stress in Rice

## TL;DR

This study investigates how different versions of a rice transporter protein handle salt stress, revealing that one variant mainly transports chloride instead of sodium.

## Contribution

The study reveals the chloride-transporting function of OsHKT1;1-V2 splice variant, contrasting with the sodium-selective full-length variant.

## Key findings

- OsHKT1;1-V2 primarily transports Cl− with weak Na+ selectivity, unlike the Na+-selective OsHKT1;1-FL.
- OsHKT1;1-V2 localizes to the plasma membrane and accumulates Cl− in oocytes.
- Nipponbare rice has higher OsHKT1;1-FL transcripts and lower OsHKT1;1-V2 transcripts under salt stress compared to Pokkali.

## Abstract

OsHKT1;1, a member of the high-affinity K+ transporter (HKT) family, plays a key role in Na+ homeostasis and salinity tolerance in rice. In our previous study, multiple potential OsHKT1;1 splicing variants were identified, as well as the full-length (FL) OsHKT1;1 transcript from the salt-tolerant rice Pokkali. However, most previous studies focused solely on the full-length protein, leaving the transport functions of splice variants largely unexamined. In this study, we focused on the splice variant OsHKT1;1-V2 and compared its function and gene expression with those of OsHKT1;1-FL. Two-electrode voltage clamp experiments using Xenopus laevis oocytes revealed that the 1st start codon of OsHKT1;1-V2 is functional to exhibit bidirectional currents in bath solutions containing NaCl. Unlike the Na+-selective feature of OsHKT1;1-FL, OsHKT1;1-V2 primarily mediated Cl− transport with weak Na+ selectivity, which was supported by the higher Cl− accumulation in OsHKT1;1-V2–expressing oocytes. Subcellular localization analyses using oocytes and Arabidopsis mesophyll cells indicated plasma membrane localization of OsHKT1;1-V2, similar to OsHKT1;1-FL. Functional assays using a yeast mutant further indicated that OsHKT1;1-FL, but not OsHKT1;1-V2, mediates Na+ uptake. The same OsHKT1;1 variants were identified in the japonica cultivar Nipponbare, and OsHKT1;1-V2 of the cultivar showed Cl− transport properties similar to the one from Pokkali. Quantitative PCR analyses revealed higher abundance of OsHKT1;1-FL transcripts in Nipponbare than in Pokkali with markedly lower OsHKT1;1-V2 levels in Pokkali under salt stress. This study provides a new insight into HKT-mediated ion homeostasis under salinity stress.