# The Multi-Target lncRNA–miRNA–mRNA TRIAD in Pancreatic Cancer Diagnosis and Therapy

## TL;DR

This paper reviews how non-coding RNAs and their regulatory networks could improve diagnosis and treatment of pancreatic cancer.

## Contribution

The paper introduces the lncRNA–miRNA–mRNA TRIAD as a novel framework for understanding gene regulation in pancreatic cancer.

## Key findings

- Non-coding RNAs like lncRNAs and miRNAs regulate key oncogenic processes in pancreatic cancer.
- The TRIAD framework integrates lncRNA, miRNA, and mRNA interactions to explain complex regulatory networks.
- ncRNAs show promise as biomarkers for early detection and targeted therapies in pancreatic cancer.

## Abstract

Pancreatic cancer (PC) is one of the most lethal malignancies worldwide, characterized by late diagnosis, aggressive progression, and limited responsiveness to current therapeutic strategies. Although extensive genomic analyses have identified key driver protein-coding genes (PCGs), therapeutic approaches targeting individual genes have shown limited clinical benefit. This limitation highlights the molecular complexity of PC, where tumor progression is governed by regulatory networks that extend beyond genetic alterations. Non-coding RNAs (ncRNAs), which constitute nearly 98% of the human genome, have emerged as regulators of gene expression in cancer. Among them, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) regulate oncogenic processes, including aberrant signaling activation, tumor microenvironment remodeling, epithelial–mesenchymal transition, immune evasion, and resistance. Beyond their independent functions, lncRNAs, miRNAs, and mRNAs form an integrated regulatory network known as the lncRNA–miRNA–mRNA TRIAD, enabling control of gene expression. Such network-based regulation provides a framework for multi-target therapeutic strategies. Moreover, the rapid responsiveness and disease-specific expression patterns of ncRNAs suggest strong potential as diagnostic and prognostic biomarkers in PC, where early detection remains challenging. This review summarizes the regulatory roles of PCGs, miRNAs, and lncRNAs in PC and highlights the lncRNA–miRNA–mRNA TRIAD as a framework for understanding gene regulatory networks.