# Potential Link Between a Disruptive CAPN6 Variant and Neurodevelopmental Disorders

## TL;DR

A disruptive variant in the CAPN6 gene is linked to neurodevelopmental disorders in a family, suggesting a potential role in fetal brain development.

## Contribution

The study identifies a novel X-linked CAPN6 variant associated with neurodevelopmental and motor coordination disorders.

## Key findings

- A disruptive CAPN6 variant was found in affected family members and undergoes nonsense-mediated mRNA decay.
- CAPN6 is expressed in placental and brain tissues, and its variants may influence VEGF activity and fetal development.
- The variant lacks a MIM phenotype code but is proposed as a candidate gene for neurodevelopmental disorders.

## Abstract

The placenta is often described as the “window to the brain” due to its crucial role in fetal neurological development. In this study, we investigated a family where the older male offspring exhibited severe neurodevelopmental and mild motor coordination disorders. His brother displayed emotional and behavioral dysregulation along with mild motor coordination disorders. The father was asymptomatic, while the mother and daughter showed mild learning disabilities. Whole exome sequencing (WES) identified a disruptive X-linked pathogenic variant, c.1088_1089del p.Asp363GlyfsTer2, within the calpain-6 (CAPN6) gene. We have submitted this variant to the ClinVar database (RCV005234146.2). The variant was found in hemizygous condition in the affected male offspring and in heterozygous condition in both the mother and daughter. As predicted, the variant undergoes nonsense-mediated mRNA decay (NMD), preventing the translation of the CAPN6 gene into a functional protein. CAPN6 is a critical gene predominantly expressed in placental and trophoblast tissues. Although its function is not well characterized, CAPN6 is also expressed in several regions of the developing brain. Recent studies have shown that genetic variants in CAPN6 significantly influence vascular endothelial growth factor (VEGF) activity, thereby affecting angiogenesis and the blood supply essential for fetal growth and development. Although CAPN6 lacks an MIM phenotype code, we hypothesize that it might be enumerated as a novel candidate gene contributing to neurodevelopmental disorders. Functional studies are imperative to elucidate the role of CAPN6 in placental function and its potential implications for neurodevelopmental processes. This work aims to inspire further research into the role of CAPN6 in placental biology and its relevance to neurodevelopmental disorders.