# SMG6’s PIN (PilT N-Terminus) Domain Is Required for Nonsense-Mediated mRNA Decay (NMD) In Vivo

## TL;DR

This study shows that the PIN domain of SMG6 is crucial for NMD in mouse embryonic stem cells and adult tissues, linking NMD to cell fate and tissue homeostasis.

## Contribution

The study introduces a mouse model with specific inactivation of SMG6’s PIN domain, revealing its essential role in NMD and cell fate determination.

## Key findings

- SMG6’s PIN domain is essential for NMD activity in embryonic stem cells and adult mouse tissues.
- Loss of the PIN domain impairs differentiation but not self-renewal of embryonic stem cells.
- PIN domain deletion in adult mice disrupts tissue homeostasis in the testis and intestine.

## Abstract

Nonsense-mediated mRNA decay (NMD) is a highly conserved RNA quality and quantity surveillance machinery in eukaryotic cells, serving as an important node in the post-transcriptional gene expression. Previous studies using the complete knockout of individual NMD factors in cells or animals reveal that NMD deficiency causes developmental defects and compromises tissue homeostasis. However, because most NMD factors participate in multiple molecular functions, a direct link between NMD and cell fate determination is missing. SMG6 is a core NMD effector and the only endoribonuclease among all NMD factors. The NMD function of SMG6 is exclusively mediated by its PIN (PilT N-terminus) domain. In this study, we engineered a mouse model with the capability of specifically deactivating the SMG6’s PIN domain/endoribonuclease activity (Smg6-PINF/F), but not knocking out the complete SMG6 protein. We found that SMG6’s PIN domain is essential for NMD activity in embryonic stem cells (ESCs) and various tissues of adult mice. Furthermore, loss of SMG6’s PIN domain is dispensable for the mouse ESC self-renewal, but severely compromises the differentiation, which consequently causes the mutant mice to die during the process of organogenesis. Through the induced deletion of SMG6’s PIN domain in adult mice, we found that loss of SMG6’s NMD function affects the homeostasis of several mouse tissues, including the testis and the intestine. In sum, our study establishes a mechanistic link between NMD per se and cell fate determination of mouse ESCs, as well as in the tissues of adult mice, where cell fate transitions are actively ongoing. The Smg6-PINF/F mouse line could be a valuable strain for elucidating the biology of NMD per se.