# High-Throughput Screening and Confirmation of 420 Hazardous Substances in Feed Based on Liquid Chromatography−High-Resolution Mass Spectrometry

## TL;DR

This paper presents a method using LC-HRMS to detect 420 hazardous substances in feed, ensuring safety and quality.

## Contribution

A validated LC-HRMS method for high-throughput screening and confirmation of 420 hazardous substances in feed.

## Key findings

- The method achieved LODs of 5–500 μg/L and SDLs of 50–500 μg/L.
- Matrix effects ranged from 36.12% to 121.16%, with precision between 0.02% and 14.98%.
- The method successfully detected hazardous substances in real feed samples.

## Abstract

Detection of hazardous substances in feed is important for ensuring human health. A method based on liquid chromatography-high-resolution mass spectrometry (LC-HRMS) was developed and validated for the screening and confirmation of 420 hazardous substances, including pesticides, veterinary drugs, and mycotoxins commonly found in feed. The screening phase employed less stringent criteria to minimize false negatives caused by matrix effects. Subsequently, stricter identification criteria were applied for confirmation to avoid false positives from interfering compounds. The performance of the proposed method was verified by limit of detection (LOD, 5~500 μg/L), screening detection limits (SDL, 50~500 μg/L), matrix effect (ME, 36.12~121.16%), precision (0.02~14.98%), stability, and accuracy. The method was successfully applied to real feed samples, demonstrating its capability to detect the presence of the 420 target hazardous substances. We believe our method provides strong technical support for ensuring the quality and safety of feed.