# Whole-genome sequencing of Bacillus pacificus B630 isolated from rice that produces biofilms

**Authors:** Luis-Daniel Sánchez-Arcos, Alberto Patricio-Hernández, Karen Cortés-Sarabia, Hugo-Alberto Rodríguez-Ruiz, Arturo Ramírez-Peralta

PMC · DOI: 10.1128/mra.00727-25 · 2026-01-26

## TL;DR

Scientists sequenced the genome of a rice-isolated Bacillus pacificus strain known for making a lot of biofilm.

## Contribution

The study identifies biofilm-related operons in Bacillus pacificus B630, linking them to known biofilm genes in Bacillus cereus.

## Key findings

- The strain was identified as Bacillus pacificus based on genomic analysis.
- The genome contains operons like sipW-tasA-calY and eps1 associated with biofilm production.
- These operons may explain the observed biofilm formation in the strain.

## Abstract

A strain from the Bacillus group isolated from rice that produced excessive biofilm on glass was identified as Bacillus pacificus. This strain has operons related to biofilm production in Bacillus cereus, such as sipW-tasA-calY and eps1, which may explain the biofilm formation.

## Linked entities

- **Genes:** sipW (type I signal peptidase) [NCBI Gene 938542], tasA (major biofilm matrix component) [NCBI Gene 938545], CALY (calcyon neuron specific vesicular protein) [NCBI Gene 50632], EPS1 (HXXXD-type acyl-transferase family protein) [NCBI Gene 836851]
- **Species:** Bacillus pacificus (taxon 2026187), Bacillus cereus (taxon 1396), Oryza sativa (taxon 4530)

## Full-text entities

- **Species:** Bacillus pacificus (species) [taxon 2026187], Bacillus cereus (species) [taxon 1396], Oryza sativa (Asian cultivated rice, species) [taxon 4530]

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Source: https://tomesphere.com/paper/PMC12896325