# Microbiota composition of fruit flies and their environments in eastern USA orchards

**Authors:** Wendy A. Cisneros Cancino, Joseph T. Gale, Aubrey Cluff, Amanda Morrison, Sarah J. Gottfredson Morgan, Maggie Nosker, Jack K. Beltz, Paul Schmidt, John M. Chaston

PMC · DOI: 10.1128/mra.01233-25 · 2026-01-12

## TL;DR

This study explores the microbial communities of fruit flies and their environments in eastern USA orchards.

## Contribution

The research identifies how microbiota composition varies with fly taxonomy, location, and environmental factors.

## Key findings

- Microbiota composition varies significantly with fly taxonomy and sampling location.
- Environmental factors like sampling substrate and starvation condition influence microbial communities.
- Key variables affect the structure of microbiota in fruit flies and their surroundings.

## Abstract

We present a 16S rRNA analysis of the microbiota of fruit flies and their fruit and soil environments collected across a latitudinal gradient in the eastern USA. Collections varied according to fly taxonomy, location, sampling substrate, and starvation condition. These samples reveal variation in microbiota composition with several key variables.

## Full-text entities

- **Species:** Drosophila melanogaster (fruit fly, species) [taxon 7227]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12896314/full.md

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Source: https://tomesphere.com/paper/PMC12896314