# Complete genome sequence of Escherichia coli EPI300

## TL;DR

This paper presents the full genome sequence of Escherichia coli EPI300, a strain useful for DNA library production and genetic studies.

## Contribution

The paper provides a closed circular reference genome for EPI300 using PacBio sequencing.

## Key findings

- The EPI300 strain has an inducible plasmid copy number control system.
- PacBio sequencing produced a complete and closed genome assembly.
- The genome is suitable for mapping and editing the EPI300 genetic system.

## Abstract

Here, we report on the complete genome sequence of Escherichia coli EPI300. This strain harbors an inducible plasmid copy number control system supporting DNA library production and functional screening applications. PacBio long-read sequencing and assembly resolved a closed circular reference genome useful for mapping and editing the EPI300 genetic operating system.