# Amplicon sequencing of ice and water phytoplankton and bacterial communities during an extreme winter in a central Canadian great lake

**Authors:** Mark J. Rozmarynowycz, Katelyn M. Brown, J. D. Gantz, Richard E. Lee, Arthur Zastepa, Sue Watson, R. Michael L. McKay

PMC · DOI: 10.1128/mra.01232-25 · 2025-12-23

## TL;DR

The study analyzed ice and water microbes in Lake Winnipeg during an extreme winter to understand community patterns.

## Contribution

It reveals how specific microbes partition into ice during cold periods in a central Canadian lake.

## Key findings

- Diatoms, cyanobacteria, and Xanthomonadales showed distinct partitioning into ice fractions.
- Cold temperatures influenced microbial community distribution in Lake Winnipeg's ice and water.

## Abstract

Ice and water microbial communities from Lake Winnipeg were explored through V4 region 16S rRNA gene sequencing during a pronounced period of cold temperatures over North America’s Great Plains and Great Lakes regions. Diatoms, cyanobacteria, and Xanthomonadales (Gammaproteobacteria) displayed patterns of partitioning into ice fractions.

## Linked entities

- **Species:** Gammaproteobacteria (taxon 1236)

## Full-text entities

- **Chemicals:** Ice (MESH:D007053)

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12896203/full.md

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Source: https://tomesphere.com/paper/PMC12896203