RsWRKY15–RsPDR12 module regulates Cd uptake and accumulation by promoting Cd efflux in radish (Raphanus sativus L.)
Shilin Ma, Zhihe Yang, Bingshuang Li, Liang Xu, Yan Wang, Yinbo Ma, Xiaoli Zhang, Jingxue Li, Kai Wang, Xinyu Zhang, Liwang Liu
TL;DR
This study identifies a gene network in radish that helps reduce cadmium accumulation by promoting its efflux from plant cells.
Contribution
The study reveals a novel regulatory module (RsWRKY15–RsPDR12) that controls cadmium efflux in radish.
Findings
RsPDR12 reduces cadmium accumulation by promoting Cd2+ efflux in plants.
RsWRKY15 activates RsPDR12 transcription and mitigates cadmium stress.
Overexpression of RsWRKY15 reduces root cadmium concentration in radish.
Abstract
Cadmium (Cd), a non-essential and toxic heavy metal, accumulates in radish taproot and adversely affects radish safety and quality. Pleiotropic drug resistance (PDR) proteins play a crucial role in heavy metal uptake and accumulation in plants. However, the PDR gene-mediated regulation of Cd accumulation remains unexplored in radish. RsPDR12 gene expression significantly increased under Cd stress. RsPDR12 demonstrated Cd transport activity and decreased Cd accumulation in yeast cells, and its overexpression reduced root Cd concentration by Cd2+ efflux in Arabidopsis. RsPDR12 alleviated Cd stress by enhancing membrane permeability and reactive oxygen species (ROS) scavenging in radish plants. RsWRKY15 was identified as the upstream regulatory factor of RsPDR12. Dual-luciferase assay demonstrated that RsWRKY15 bound to the RsPDR12 promoter to activate its transcription. RsWRKY15…
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Taxonomy
TopicsPlant Gene Expression Analysis · Plant Stress Responses and Tolerance · Aluminum toxicity and tolerance in plants and animals