CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance Separation of Human Mesenchymal Stromal Cells
Moe Kato, Tadashi Nakaji‐Hirabayashi, Kazuaki Matsumura, Chiaki Yoshikawa, Yuki Usui, Takahiro Kishioka, Taito Nishino
TL;DR
A new polymer surface with a special peptide can safely and efficiently separate human mesenchymal stem cells without using labels.
Contribution
A peptide-functionalized antifouling polymer system for label-free, selective isolation of hMSCs is developed.
Findings
The PC7P2M1–CD44BP surface effectively suppressed nonspecific protein adsorption and nontarget cell adhesion.
The system achieved high-purity hMSC isolation within 35 minutes while preserving cell function.
Isolated hMSCs maintained proliferation and differentiation capacities similar to pre-separated cells.
Abstract
Cell transplantation therapy is a promising strategy for next‐generation regenerative medicine. However, its clinical application is limited by the absence of safe and label‐free methods for obtaining pure populations of functional cells. This study reports a peptide‐functionalized zwitterionic polymer system designed for the selective and noninvasive isolation of human mesenchymal stem cells (hMSCs) from heterogeneous cell populations. The substrate was modified with poly(CMBMAm‐co‐PGMAn‐co‐MPTMS1) (PC m P n M1), which was synthesized via free radical polymerization, and subsequently conjugated with a CD44‐binding peptide (CD44BP). Among the compositions examined, the PC7P2M1–CD44BP surface exhibited superior antifouling properties, effectively suppressing nonspecific protein adsorption and adhesion of nontarget cells while selectively capturing hMSCs. A column packed with…
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Taxonomy
TopicsPolymer Surface Interaction Studies · Hydrogels: synthesis, properties, applications · Electrospun Nanofibers in Biomedical Applications