# Low-temperature synthesis of di- and monoolein by enzymatic hydrolysis in a biphasic system using cutinase from Fusarium graminearum

## TL;DR

Researchers developed a low-temperature method to produce beneficial oleoylglycerols using an enzyme from Fusarium graminearum in a biphasic system.

## Contribution

The study introduces a novel enzymatic approach using cutinase from Fusarium graminearum for low-temperature synthesis of specific oleoylglycerols.

## Key findings

- Cutinase from Fusarium graminearum (FGC) shows optimal activity at low temperatures (2–10°C) and weak alkaline conditions (pH 8–9).
- FGC produces only 2-monoolein with 15.7 ± 3.83% conversion at 15% hydrolysis of triolein.

## Abstract

Among functional lipids, oleoylglycerols including 1,2-diolein and 2-monoolein are known for their beneficial activities such as fatty acid metabolism, insulin secretion promotion, antioxidant, and anti-arrhythmic sclerosing activity. To produce these oleoylglycerols through the lipase reaction, cutinase from Fusarium graminearum (FGC) were recombinantly expressed and the properties of the enzyme were analyzed. The enzyme exhibited optimal activity under weak alkaline conditions (pH 8–9) and low temperatures (2–10 °C) for triolein in the water–isooctane biphasic system. The product of the lipase reaction with FGC at a low temperature was analyzed by HPLC, revealing that FGC appeared as sn-1(3) regiospecific lipase and only 2-monoolein was produced in monoolein isomers with 15.7 ± 3.83% conversion at 15% hydrolysis of triolein. These results showed that the FGC can be used to produce 1,2-diolein and 2-monoolein and further suggest its potential as a versatile enzyme in lipid modification in low-temperature environments.

The online version contains supplementary material available at 10.1007/s10068-025-02062-z.