# Pseudidiomarina xizangensis sp. nov. and Terrihabitans aquatilis sp. nov., isolated from LungmuCo lake in Xizang, with the reclassification of Flaviflagellibacter deserti as Terrihabitans deserti comb. nov

## TL;DR

Two new bacterial species were discovered in a lake in Xizang, China, and one previously named species was reclassified.

## Contribution

The paper introduces two novel bacterial species and reclassifies an existing one using genetic and biochemical evidence.

## Key findings

- Strain E22-M8T is a new species named Pseudidiomarina xizangensis.
- Strain B22-R8T is a new species named Terrihabitans aquatilis.
- Flaviflagellibacter deserti was reclassified as Terrihabitans deserti.

## Abstract

Two bacterial strains, designated E22-M8T and B22-R8T, were isolated from water sample collected from LungmuCo lake in Xizang of China. The 16S rRNA gene analysis revealed that strain E22-M8T was most closely related to members of the genus Pseudidiomarina, exhibiting the highest sequence similarity of 97.38 and 97.39% to Pseudidiomarina halophila BH195T and Pseudidiomarina terrestris 1APP75-27aT, respectively. While strain B22-R8T showed the highest similarity to species of the genera Terrihabitans and Flaviflagellibacter: 97.69, 96.80 and 96.66% to ‘Terrihabitans rhizophilus’ PJ23T, Terrihabitans soli IZ6T and Flaviflagellibacter deserti SYSU D60017T. For strain E22-M8T, the digital DNA–DNA hybridization (dDDH) values with its closest relatives P. halophila BH195T and P. terrestris 1APP75-27aT were 25.7 and 20.1%, respectively; the average nucleotide identity (ANI) values were 83.5 and 78%; and the average amino acid identity (AAI) values were 90.4 and 86.4%, all below the recognized species delineation thresholds. For strain B22-R8T, the corresponding dDDH, ANI and AAI values with its closest relatives ‘T. rhizophilus’ PJ23T, T. soli IZ6T and F. deserti SYSU D60017T also fell below the respective species thresholds. Biochemically and physiologically, both strains exhibited distinct traits that further supported their novelty. Based on comprehensive polyphasic analyses, strain E22-M8T (=CGMCC 1.19205T=KCTC 92346T) is proposed as a novel species named Pseudidiomarina xizangensis sp. nov., and strain B22-R8T (=CGMCC 1.19187T=KCTC 92343T) as a novel species designated Terrihabitans aquatilis sp. nov.