Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity
Samah H. Abu-Hussien, Akebe Luther King, Muhammad A. Khan
TL;DR
This study creates copper oxide nanoparticles from thyme that effectively fight drug-resistant bacteria and show anticancer properties.
Contribution
The study introduces a novel green-synthesis method for copper oxide nanoparticles with optimized antimicrobial and anticancer efficacy.
Findings
TE-CuONPs showed strong synergy with gentamicin, reducing required doses by eightfold against S. aureus and P. aeruginosa.
The nanoparticles induced apoptosis in 77.25% of MCF-7 breast cancer cells and had threefold higher antioxidant activity than the plant extract.
Optimized synthesis produced monodisperse CuO nanoparticles with MIC values of 250–950 μg/mL against multidrug-resistant bacteria.
Abstract
The dual crises of antimicrobial resistance and cancer demand innovative therapeutic platforms that overcome conventional treatment limitations. This study uniquely combines systematic Box-Behnken optimization of green-synthesized copper oxide nanoparticles from Thymus vulgaris with comprehensive evaluation of their synergistic antimicrobial and anticancer activities. HPLC profiling identified quercetin (55.92%), chlorogenic acid (15.33%), and gallic acid (12.28%) as principal phytochemical reducing and capping agents. Statistical optimization (R2 = 0.9886) established copper acetate concentration (F = 670.48, p < 0.0001) and incubation time (F = 124.11, p < 0.0001) as critical synthesis determinants, yielding monodisperse spherical nanoparticles (19–25 nm TEM; Z-average 119.2 nm, PDI 0.22; ζ-potential − 45.8 mV). XRD confirmed a crystalline monoclinic CuO phase, while FTIR validated…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 10
Figure 10
Figure 10
Figure 11
Figure 1
Figure 2
Figure 3
Figure 3
Figure 4
Figure 5
Figure 5
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 9
Figure 20Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsNanoparticles: synthesis and applications · Copper-based nanomaterials and applications · Gold and Silver Nanoparticles Synthesis and Applications