# Polyphasic characterization of Nocardioides aquaegermanicae sp. nov., a novel water-derived actinobacterium

## TL;DR

Scientists discovered a new species of water-derived bacteria, Nocardioides aquaegermanicae, based on genetic and chemical analysis.

## Contribution

A novel species of actinobacterium is described with distinct genomic and chemotaxonomic features.

## Key findings

- The strain DSM 117947T has less than 70% DNA-DNA hybridization and 95-96% ANI with its closest relative.
- The polar lipid profile and fatty acid composition distinguish the strain from related species.
- Genomic G+C content of 73% and menaquinone MK-8(H4) are key features of the new species.

## Abstract

Strain DSM 117947T was isolated from a Micromonospora matsumotoense co-culture originating from a water sample collected in Germany. The strain was subjected to a polyphasic taxonomic analysis. It exhibited 99.3% 16S rRNA gene sequence similarity with Nocardioides aurantiacus DSM 12652T. Digital DNA-DNA hybridization and average nucleotide identity values between the strain and its close phylogenetic neighbour were below the threshold of 70% and 95−96% for prokaryotic species demarcation, respectively. The strain had a polar lipid profile composed of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmethylethanolamine (PME), phosphatidylinositol (PI), glycophospholipid (GPL), and phospholipids (PLs). The predominant menaquinone (>20%) was MK-8(H4). The major fatty acids (>5%) were C16.0, C16:1 ω7c, and C18:1 ω9c. The genomic G + C content of the strain is 73%. The chemotaxonomic, biochemical, enzymatic, and genomic features distinguished the strain from its close relative, and justify its assignment to a novel species, for which the name Nocardioides aquaegermanicae sp. nov., is proposed, with strain DSM 117947T (WG_orangeT = KCTC 59414T) as the type strain.