# Pseudopaline-mediated zinc uptake by Pseudomonas aeruginosa drives clinically relevant phenotypes and infection outcomes

## TL;DR

This study shows that Pseudomonas aeruginosa uses a special system called pseudopaline to get zinc when it's scarce, which is crucial for its growth and causing infections.

## Contribution

The study identifies pseudopaline as the last-resort zinc uptake pathway in Pseudomonas aeruginosa under metal-restricted conditions.

## Key findings

- Pseudopaline is essential for Pseudomonas aeruginosa to acquire zinc when other pathways fail.
- Pseudopaline-mediated zinc uptake supports clinically relevant phenotypes like biofilm formation and antibiotic tolerance.
- The pseudopaline system is critical for Pseudomonas aeruginosa pathogenicity and infection outcomes.

## Abstract

Biological metals are vital trace elements required by metalloproteins, which are involved in virtually every cellular, structural, and catalytic function of the bacterial cell. Bacterial pathogenesis involves a tug-of-war between the host’s nutritional immunity sequestering essential metals and the invading pathogens that deploy adapted high-metal affinity uptake strategies, such as metallophores, in order to efficiently circumvent these defense mechanisms. Pseudopaline is a metallophore produced and secreted by Pseudomonas aeruginosa to acquire zinc when the bioavailability of this metal is severely restricted, as in the presence of a strong metal chelator such as EDTA, or during infections when the nutritional immunity of the host is active. We show that when facing strong metal chelation, the general Znu zinc uptake pathway becomes ineffective and only the pseudopaline pathway is capable of supplying the bacteria with the necessary zinc to maintain their growth, establishing that the pseudopaline pathway is the last-resort pathway for the bacteria to acquire zinc under such restricted growth conditions. Based on this statement, the present study explores the pleiotropic role of pseudopaline-mediated zinc acquisition on clinically relevant phenotypes such as biofilm formation and associated antibiotic tolerance, as well as its capacity to determine infection outcomes using cell-culture and murine models. The expression of pseudopaline-dependent phenotypes in such a diversity of biological contexts demonstrates the essentiality of this specific metal uptake system for P. aeruginosa pathogenicity during infection. We therefore identify this machinery as a promising therapeutic target for P. aeruginosa infections.