# Alcohol Oxidase–Imine Reductase Cascade for One-Pot Chiral Amine Synthesis

## TL;DR

This paper introduces a new one-pot method using enzymes to efficiently produce chiral amines from allylic alcohols.

## Contribution

A novel biocatalytic cascade using Alcohol Oxidase and Imine Reductase for streamlined chiral amine synthesis.

## Key findings

- The cascade method works under mild conditions and handles a wide range of substrates.
- The tandem enzymatic system outperforms traditional stepwise methods in selectivity and efficiency.
- The system shows substrate-dependent selectivity, enhancing the synthesis of enantioenriched amines.

## Abstract

We report a one-pot
biocatalytic cascade for the conversion of
allylic alcohols into enantioenriched secondary amines through a sequence
involving oxidation followed by conjugate reduction–reductive
amination (CR–RA). This transformation, catalyzed by a cholesterol
oxidase (ShCO) and an ene-imine reductase (EneIRED), proceeds under
mild conditions and accommodates a broad substrate scope. In several
cases, the cascade outperforms the stepwise method and exhibits substrate-dependent
selectivity, highlighting the advantages of tandem enzymatic systems
for efficient and streamlined chiral amine synthesis.