# Discovery of Widespread Migrasome Formation During Amoeboid Migration in Dictyostelium discoideum

## TL;DR

The study shows that migrasomes form during cell migration in Dictyostelium and may help with communication and development.

## Contribution

The discovery of migrasome formation during amoeboid migration in Dictyostelium discoideum is novel.

## Key findings

- GFP-CpnE labels retraction fibers and migrasomes during chemotaxis and migration.
- Migrasomes contain RNA and are stained by WGA and SYTO RNASelect.

## Abstract

Migrasomes are organelles that form along retraction fibers of migrating cells and mediate intercellular communication. We performed localization studies on the calcium-dependent phospholipid-binding protein, copine E (CpnE), in
Dictyostelium discoideum
. We observed GFP-CpnE labeled retraction fibers and migrasomes in multiple contexts: chemotaxis toward folate, random migration, and early development. Migrasome membranes were stained with WGA and some migrasomes were stained with SYTO RNASelect, indicating the presence of RNA. Our findings suggest that CpnE may have a role in migrasome formation and indicate that migrasomes may play important roles in
Dictyostelium
development, cell communication, and/or homeostasis in a variety of environments.