# Creating large 60Co-γ populations for functional genomics and breeding in wheat

## TL;DR

This study uses gamma radiation to create a large wheat mutant population for improving traits like freezing and salt tolerance.

## Contribution

A large-scale wheat mutant population with diverse traits is developed for functional genomics and breeding.

## Key findings

- 158 freezing-tolerant wheat mutants were identified.
- 441 saline-alkali-tolerant mutants were found.
- Over 5,000 mutants showed changes in yield or quality traits.

## Abstract

Wheat (Triticum aestivum L.) serves as a critically important staple crop worldwide, and mutation breeding through Cobalt-60 (60Co-γ) radiation has been widely adopted as an effective strategy for genetic improvement. In this study, ten wheat cultivars from Shandong, Henan, and Hebei were subjected to 60Co-γ irradiation to develop an M2 mutant population comprising 10,350,000 lines. Systematic screening M2 mutant population under natural conditions identified 158 freezing-tolerant mutants, 441 saline-alkali-tolerant mutants, and >5,000 mutants with changed yield or quality traits. This population represents a valuable genetic resource for collaborative research and provides a powerful platform for functional genomics studies and breeding applications.