# Evolution of Multivalent Aptamer Corona for High‐Throughput Multiplexed Detection of Multiple Cancers

## TL;DR

A new method using aptamer coronas enables high-throughput detection of multiple cancers with high accuracy using clinical serum samples.

## Contribution

A multivalent aptamer corona platform is introduced for multiplexed, high-throughput cancer detection using a novel ProteoFish-SELEX strategy.

## Key findings

- Aptamer coronas for ovarian, lung, and colorectal cancers were successfully evolved using ProteoFish-SELEX.
- High diagnostic accuracy and specificity were achieved in multiplexed cancer detection using clinical serum samples.
- The platform enables sequencing-enabled high-throughput analysis and identifies cancer-specific protein signatures.

## Abstract

Clinical in vitro diagnosis is essential in early diagnosis and prognostic assessment, yet antibody‐based assays face limitations in multiplexity and scalability. To address the growing demand for modern molecular diagnostics, a multivalent aptamer corona platform for high‐throughput is introduced and multiplexed multi‐cancer diagnosis. With clinical serum samples, ovarian cancer‐, lung cancer‐, and colorectal cancer‐based aptamer coronas are obtained through several rounds of alternating positive and negative systematic evolution of ligands by exponential enrichment (SELEX). Leveraging a de novo ProteoFish‐SELEX strategy, which integrates nanoparticle‐protein corona technology, a multivalent aptamer corona is evolved. Proteomic profiling of protein coronas revealed cancer‐associated protein signatures, while aptomic profiling of aptamer coronas identified high‐affinity cancer‐specific aptamers. With these aptamers, high diagnostic accuracy is demonstrated in multiplexed multi‐cancer detection using clinical cohorts. Multivalent aptamer corona's outstanding diagnostic performance, multiplexed detection capability, and sequencing‐enabled high‐throughput potential position it as a promising versatile tool for novel biomarker discovery and a transformative advancement in precision oncology.

A multivalent aptamer corona is evolved via established ProteoFish‐SELEX (systematic evolution of ligands by exponential enrichment) for high‐throughput multiplexed detection of multiple cancers. Clinical validation using multivalent aptamer corona demonstrated high accuracy and specificity in multiplexed detection of ovarian, lung, and colorectal cancers using serum samples from different cohorts.