# Single‐Cell Profiling: Any Scale, Any Size, All at Once

## TL;DR

New single-cell technologies overcome past limitations by enabling high-throughput, large-scale, and multi-modal profiling of cells, improving understanding of cellular identity and function.

## Contribution

This paper introduces three recent single-cell technologies that enhance throughput, resolution, and sensitivity for multi-modal profiling.

## Key findings

- Recent innovations allow high-throughput profiling of large-sized cells.
- Multi-modal profiling of patient samples is now possible with improved resolution and sensitivity.
- The described technologies overcome limitations in preserving spatial information.

## Abstract

Single‐cell technologies have revolutionized the understanding of cellular heterogeneity, revealing distinct cell populations and functional states with molecular precision. Traditional approaches, however, are constrained by cellular dimension and throughput scale, in addition to its inability preserve spatial information. Recent innovations now allow high throughput, large‐sized cell, and multi‐modal profiling of patient samples, overcoming these limitations. In this perspective, three recently developed single‐cell technologies is described, highlighting how their enhanced throughput, resolution, and sensitivity provide deeper insights into cellular identity and function.

Traditional single‐cell technologies are constrained by cellular dimension and throughput scale, in addition to its inability preserve spatial information. With recent innovations, these technologies now allow high throughput, large‐sized cell, and multi‐modal profiling of patient samples, overcoming these limitations. Here, we describe three recently developed single‐cell technologies, highlighting how their enhanced throughput, resolution, and sensitivity provide deeper insights into cellular identity and function.