# Wild Prunus cerasifera Ehrh. Polyphenols Alleviate Hyperglycemia in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota‐SCFAs‐PI3K/Akt/TBC1D4 Pathway

## TL;DR

Wild Prunus cerasifera polyphenols reduce blood sugar in diabetic mice by improving gut bacteria and liver function.

## Contribution

This study reveals a new dietary approach for managing type 2 diabetes through gut microbiota and liver pathway modulation.

## Key findings

- WPPE treatment lowered blood glucose and improved insulin resistance in T2DM mice.
- WPPE increased short-chain fatty acids and regulated T2DM-related gut bacteria.
- WPPE activated the PI3K/AKT/TBC1D4 pathway to enhance glycogen synthesis.

## Abstract

Our previous work demonstrated that wild
Prunus cerasifera
Ehrh. possesses notable in vitro hypoglycemic activity. This study further evaluated the effects of wild
Prunus cerasifera
Ehrh. polyphenol extract (WPPE) on T2DM mice. WPPE treatment significantly lowered blood glucose, improved insulin resistance, and reduced hepatic oxidative stress and inflammation in T2DM mice. It could promote the production of short‐chain fatty acids and regulate the relative abundance of T2DM‐associated bacteria like Muribaculaceae and Odoribacter, and enhance hepatic glycogen synthesis through activation of the key gene involved in the PI3K/AKT/TBC1D4 pathway, resulting in hypoglycemic effect based on the gut‐liver axis. These findings support WPPE as a promising dietary candidate for T2DM management.

WPPE treatment significantly alleviated type 2 diabetes by improving SCFAs and gut microbiota composition and promoted glycogen synthesis by regulating the PI3K/AKT/TBC1D4 pathway. WPPE also improved liver function and reduced oxidative stress and inflammation.