# Artificial thymic organoid culture generates functional iPSC-derived CD4+ invariant natural killer T cells

## TL;DR

Scientists created functional CD4+ iNKT cells from iPSCs using a 3D organoid system, which could help boost anti-tumor immunity.

## Contribution

A novel 3D organoid culture method successfully generates functional CD4+ iNKT cells from iPSCs.

## Key findings

- 3D-CD4+ iNKT cells exhibit antigen-specific helper functions and produce IFN-γ/IL-4.
- They induce dendritic cell maturation and reverse T cell suppression by immunosuppressive macrophages.
- These cells may serve as an adjuvant T-cell source for enhancing anti-tumor immunotherapy.

## Abstract

Invariant NKT (iNKT) cells have a T-cell receptor that is common to all individuals and are activated by recognizing glycolipids on MHC class I-like CD1d molecules. Activated iNKT cells are known to exert anti-tumor effects through the activation of other immune cells and have attracted attention as promising T cells for eliciting anti-tumor immunity. However, securing a sufficient number of iNKT cells is an obstacle to treatment because iNKT cells are a very small cell population, less than 0.1% of the peripheral blood lymphocytes. Although previous studies have demonstrated redifferentiation of a large number of CD4–CD8– double-negative iNKT cells from induced pluripotent stem (iPS) cells in two-dimensional monolayer cultures, CD4+ single-positive (CD4SP) iNKT cells could not be induced. Here we show CD4SP iNKT cells can be obtained by three-dimensional (3D) organoid culture (3D-CD4+ iNKT cell). We additionally describe 3D-CD4+ iNKT cells show antigen-specific helper functions, as they proliferate, produce interferon-γ/interleukin-4 (IFN-γ/IL-4), and induce dendritic cell maturation in response to α-galactosylceramide. Furthermore, they reverse the inhibition of T cell proliferation induced by immunosuppressive macrophages in an antigen-specific manner. Collectively, 3D-CD4+ iNKT cells may become an adjuvant T-cell source to enhance current T-cell immunotherapy against solid tumors.

Functional CD4+ invariant Natural Killer T cells were induced from iPSCs using the Artificial Thymic Organoid method. It showed antigen specific adjuvant function and ability to release T cell suppression by M2 macrophage.