# Transport stress induces paradoxical increases in airway inflammatory responses in beef stocker cattle

## TL;DR

Transport stress in beef calves causes increased lung inflammation unrelated to infection, which could explain how stress contributes to respiratory disease.

## Contribution

This study shows that transport stress directly triggers inflammatory immune responses in the lungs of beef calves, independent of pathogen exposure.

## Key findings

- Stressed calves showed upregulation of inflammatory genes like CXCL8, CSF3R, IL1B, and CCL22.
- Stressed calves had significantly higher neutrophil levels in lung fluid compared to acclimated calves.
- Neutrophilic infiltration occurred without pathogen colonization in most calves.

## Abstract

The development of Bovine Respiratory Disease (BRD) in beef cattle is associated with stressful events, including auction and transport. In addition to the effects of commingling on pathogen exposure, stress also impacts immune function and has classically been associated with an immunosuppressed state. Much of the research on cattle immunity in stress has focused on peripheral blood immune functionality rather than mucosal immune responses. To characterize immunity at the primary site of pathogen colonization in BRD, we evaluated stress responses in lung cells from beef stocker cattle to understand mucosal immune changes. Stocker calves were sampled via bronchoalveolar lavage fluid (BALF) collection within 24 hours of auction and transport to a new facility (Stressed, n = 12), or allowed to acclimate for 2 months at the new facility before sampling (Acclimated, n = 7). Lavage cellular RNA was extracted and sequenced for bulk RNA-seq gene expression. Differential gene expression analysis of RNA sequencing data demonstrated a profound upregulation of inflammatory genes in Stressed calves compared to Acclimated calves, including expression of CXCL8, CSF3R, IL1B, and CCL22. Top pathway upregulation in Stressed calves involved neutrophil migration and chemotaxis, and cytokine signaling. To predict cellular population proportions from the data, CIBERSORTx was used to deconvolute bulk RNA-seq gene counts. This analysis showed that Stressed calves had significantly increased BALF neutrophils compared to Acclimated calves (p = 0.003). Neutrophilic infiltration occurred in the absence of pathogen colonization of the lungs in most calves, as demonstrated by a multi-pathogen respiratory qPCR screen. As such, the stress induced an inflammatory response in lungs not explained by pathogen exposure. This study provides strong evidence that shipping stress in beef stocker calves can trigger increased inflammatory pulmonary mucosal immune responses, which has important implications for the pathogenesis of BRD.