# Cellulases: key properties, natural sources, and industrial applications

**Authors:** A.V. Zadorozhny, N.M. Slynko, S.V. Bannikova, N.V. Bogacheva, V.N. Shlyakhtun, A.R. Vasilieva, E.Yu. Bukatich, V.S. Ushakov, Yu.E. Uvarova, A.V. Korzhuk, A.A. Shipova, D.V. Bochkov, E.Y. Pavlova, D.O. Chesnokov, S.E. Peltek

PMC · DOI: 10.18699/vjgb-25-141 · Vavilov Journal of Genetics and Breeding · 2025-12-01

## TL;DR

This paper reviews cellulases, enzymes that break down cellulose, focusing on their properties, sources, and industrial uses.

## Contribution

The paper provides a comprehensive overview of cellulases, emphasizing extremophilic variants and their industrial relevance.

## Key findings

- Cellulases are crucial for breaking down cellulose in various industries.
- Extremophilic cellulases are highlighted for their suitability in modern industrial processes.
- The paper discusses methods for producing and modifying cellulases for practical applications.

## Abstract

This review focuses on cellulases, a subclass of hydrolases that catalyse the breakdown of the polysaccharide cellulose. Cellulases are of immense practical significance, given that cellulose-containing materials are utilised across a multitude of industrial sectors. An overview of the fundamental properties and structure of cellulases is provided. However, primary attention is paid to the industrial application of these enzymes, with other aspects discussed within this context. The most practically significant bacterial and fungal cellulases are analysed, with their key benefits and differences being emphasised. Particular attention is paid to extremophilic (specifically thermo-, psychro-, and halophilic) cellulases, as they possess properties essential for modern technological processes. Given that practical application necessitates mass production and an optimal combination of enzymatic characteristics, the creation of effective producers and the modification of cellulase properties are also assessed. Finally, key trends in cellulase production approaches and their future application potential are summarised.

## Full-text entities

- **Genes:** Sso7d [NCBI Gene 98910198], protein disulfide [NCBI Gene 8319091], endoglucanase [NCBI Gene 29358324], phospholipase [NCBI Gene 29358208], beta-glucosidase [NCBI Gene 29361070]
- **Diseases:** toxicity (MESH:D064420), rheumatoid arthritis (MESH:D001172), metabo- lic disorders (MESH:D009358)
- **Chemicals:** Cellulose (MESH:D002482), oligosaccharides (MESH:D009844), taxol (MESH:D017239), volatile fatty acids (MESH:D005232), fat (MESH:D005223), Ni (MESH:D009532), palm oil (MESH:D000073878), n-butanol (MESH:D020001), beta-glucans (MESH:D047071), lignin (MESH:D008031), metal (MESH:D008670), hemicellulose (MESH:C007916), Antioxid Redox (-), Na+ (MESH:D012964), sugars (MESH:D000073893), glucose (MESH:D005947), xylan (MESH:D014990), salt (MESH:D012492), fatty acid (MESH:D005227), cellodextrins (MESH:C068994), rice bran oil (MESH:D000073879), nitrogen (MESH:D009584), polysaccharide (MESH:D011134), hydrogen (MESH:D006859), oil (MESH:D009821), lignocellulose (MESH:C036909), (3R-cis)- 3-(acetyloxy)-4-phenyl-2-azetidinone (MESH:C089855), brine (MESH:C017082), water (MESH:D014867), NaCl (MESH:D012965), steviosides (MESH:C012043), carbohydrate (MESH:D002241), acid (MESH:D000143), graphite (MESH:D006108), GABA (MESH:D005680), cellobiose (MESH:D002475), methane (MESH:D008697), Disulfide (MESH:D004220), carbon (MESH:D002244), Tween-80 (MESH:D011136), phosphate (MESH:D010710), N-methyl-N'-nitro-N-nitrosoguanidine (MESH:D008769), carboxymethyl cellulose (MESH:D002266), polymer (MESH:D011108), ethanol (MESH:D000431)
- **Species:** Penicillium chrysogenum (species) [taxon 5076], Thermothelomyces thermophilus (species) [taxon 78579], Thermotoga naphthophila [taxon 93930], Rasamsonia emersonii (species) [taxon 68825], Gallus gallus (bantam, species) [taxon 9031], Siphonobacter aquaeclarae (species) [taxon 563176], Paracoccus sulfuroxidans (species) [taxon 384678], Dickeya chrysanthemi (species) [taxon 556], Geobacillus sp. (species) [taxon 1891658], Corbicula japonica (species) [taxon 141464], Trichoderma reesei (species) [taxon 51453], Actinopterygii (fishes, superclass) [taxon 7898], Ganoderma lucidum (species) [taxon 5315], Brucella cytisi (species) [taxon 407152], Paenalicyclobacillus cellulosilyticus (species) [taxon 1003997], Hominimerdicola alba (species) [taxon 1264], Clostridium cellulovorans (species) [taxon 1493], Pyrus communis (pear, species) [taxon 23211], Sodiomyces alcalophilus JCM 7366 (strain) [taxon 591952], Labrys neptuniae (species) [taxon 376174], Brachionus plicatilis (species) [taxon 10195], Pycnanthus angolensis (species) [taxon 224864], activated sludge metagenome (species) [taxon 942017], Stevia rebaudiana (species) [taxon 55670], Saccharolobus shibatae (species) [taxon 2286], Pseudomonas coleopterorum (species) [taxon 1605838], Pseudoalteromonas sp. (species) [taxon 53249], Aspergillus fumigatus Z5 (strain) [taxon 1437362], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Jatropha (genus) [taxon 3995], Bacteria Latreille et al. 1825 (Bacteria stick insect, genus) [taxon 629395], Bacillus subtilis (species) [taxon 1423], Nocardiopsis sp. (species) [taxon 310350], Saccharolobus solfataricus (species) [taxon 2287], Caldicellulosiruptor bescii (species) [taxon 31899], Euphausia pacifica (North Pacific krill, species) [taxon 102976], Anaerobacterium chartisolvens (species) [taxon 1297424], Zymomonas mobilis (species) [taxon 542], Kaistia adipata (species) [taxon 166954], Malus domestica (apple, species) [taxon 3750], Bacillus sp. (in: firmicutes) (species) [taxon 1409], Brucella haematophila (species) [taxon 419474], Gloeophyllum trabeum (species) [taxon 104355], Cellulomonas gilvus (species) [taxon 11], Sodiomyces alcalophilus (species) [taxon 398408], Citrobacter freundii (species) [taxon 546], Acetivibrio cellulolyticus (species) [taxon 35830], Dictyoglomus turgidum (species) [taxon 513050], Solanum tuberosum (potatoes, species) [taxon 4113], Ruminiclostridium cellulolyticum (species) [taxon 1521], Clostridia (class) [taxon 186801], Halobacillus (genus) [taxon 45667], Shewanella sp. (species) [taxon 50422], Caldibacillus (genus) [taxon 1276290], Sorghum bicolor (broomcorn, species) [taxon 4558], Streptomyces lividans (species) [taxon 1916], Streptomyces abietis (species) [taxon 1227734], Pseudobacteroides cellulosolvens (species) [taxon 35825], Paenibacillus sp. (species) [taxon 58172], Caldicellulosiruptor changbaiensis (species) [taxon 1222016]
- **Mutations:** G415P, Cysteine amino acid residues at positions 99

## Full text

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Source: https://tomesphere.com/paper/PMC12876952