Candidate Biomarkers for Crohn’s Disease: Hub Genes and Regulatory miRNAs Identified by Bioinformatics Analysis
Han Wang, Mengdie Shen
TL;DR
This study identifies three genes and two microRNAs that may help diagnose and treat Crohn’s disease using bioinformatics methods.
Contribution
The study proposes novel hub genes and regulatory miRNAs as potential biomarkers for Crohn’s disease.
Findings
Sixty differentially expressed genes were identified across three datasets, with 44 upregulated and 16 downregulated.
Three hub genes (CXCL1, CXCL2, CXCR2) and two miRNAs (hsa-miR-1-3p, hsa-miR-335-5p) were identified as key players in CD.
The miRNA-hub gene regulatory network suggests these genes and miRNAs could be used as diagnostic or therapeutic targets.
Abstract
Crohn’s disease (CD) is a chronic, complex inflammatory condition that can affect the entire digestive tract, most commonly the terminal ileum. The exact cause of CD remains unknown. Bioinformatics was used in this study to identify the differentially expressed genes (DEGs) and microRNAs (miRNAs) that show potential as diagnostic and therapeutic agents in treating CD. Datasets were downloaded from the Gene Expression Omnibus database and filtered. DEGs between CD samples and healthy control samples were identified using the GEO2R tool (including GEOquery and Linear Models for Microarray Analysis), and Kyoto Encyclopedia of Genes and Genomes/Gene Ontology enrichment analyses were conducted as part of the study to gain deeper insights into the data. A network depicting protein–protein interactions was established and visualized using the STRING database and Cytoscape software, and hub…
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Taxonomy
TopicsInflammatory Bowel Disease · Spondyloarthritis Studies and Treatments · Barrier Structure and Function Studies