Loss of Snhg5 disrupts cell-cycle regulation without altering cystogenesis in a mouse model of polycystic kidney disease

TL;DR

This study finds that the lncRNA Snhg5 affects cell cycle regulation in kidney cells but does not play a key role in causing kidney cysts in a mouse model of polycystic kidney disease.

Contribution

The study reveals Snhg5's role in cell cycle regulation and clarifies its dispensability in cyst formation in polycystic kidney disease.

Findings

Snhg5-null mice showed altered gene expression related to cell cycle progression and DNA replication.

Loss of Snhg5 did not reduce cyst formation in a mouse model of polycystic kidney disease.

Reduced ARPC5 levels in Snhg5-null cells suggest a link to cell cycle disruption.

Abstract

Long non-coding RNAs (lncRNAs) regulate diverse cellular pathways and are increasingly linked to human disease. Snhg5 is frequently described as a pathogenic lncRNA in many human diseases, including cancer. Our previous studies revealed that Snhg5 is one of the most upregulated lncRNAs in multiple mouse models of polycystic kidney disease (PKD). Yet its role in renal biology and in autosomal dominant PKD (ADPKD) is not known. To elucidate the role of Snhg5, we generated a global Snhg5-null mouse. Homozygous animals were viable and displayed normal kidney morphology and function. RNA-sequencing of Snhg5-null kidneys and renal epithelial cells revealed common alterations in gene expression linked to cell cycle progression and DNA replication. At the molecular level, Snhg5-null cells showed increased sub-G1 and S/G2/M fractions, coinciding with depletion of ARPC5-a core ARP2/3 subunit-suggesting that reduced ARPC5 may contribute to this phenotype. To determine whether Snhg5 upregulation is pathogenic in mouse PKD, we crossed Snhg5-null mice with a collecting duct-specific Pkd1-mutant mouse model. Loss of Snhg5 did not attenuate cyst formation; if anything, disease severity was mildly but not significantly exacerbated. These findings indicate that Snhg5 modulates cell-cycle control and is dispensable for kidney development and cystogenesis in collecting duct-derived cysts.

The online version contains supplementary material available at 10.1038/s41598-026-35234-w.

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