# Genome sequencing, de novo assembly and annotation of the commercially important bamboo, Bambusa tulda Roxb

**Authors:** Sutrisha Kundu, Oliver Rupp, Sonali Dey, Mridushree Basak, Sudeshna Bera, Annette Becker, Malay Das

PMC · DOI: 10.1038/s41597-026-06679-5 · 2026-02-04

## TL;DR

This paper presents the genome sequencing and assembly of Bambusa tulda, a commercially important bamboo species, providing a valuable resource for future research.

## Contribution

The study provides the first de novo genome assembly of Bambusa tulda using long-read sequencing, offering insights into its genetic structure and function.

## Key findings

- The genome assembly consists of 43 contigs across three subgenomes with a total length of 1.37 Gb.
- Functional annotation identified 56,890 protein-coding genes, covering 19.44% of the genome.

## Abstract

Bambusa tulda Roxb., a member of the Bambusoideae subfamily, is an ecologically and commercially important plant resource widely distributed in the Indian subcontinent. Our study reports long-read PacBio HiFi sequencing and genome assembly of B. tulda. The de novo haploid genome assembly of B. tulda predicted 43 contigs, distributed across three subgenomes, with a total sequenced length of 1.37 Gb, contig N50 of 35.69 Mb, and BUSCO score 99%. Repetitive elements constitute 63.31% of the genome. Functional annotation predicted 56,890 protein-coding genes, constituting 19.44% of the genome. This genome sequence will serve as an invaluable resource for future studies on the life history traits, phylogenomic analysis, comparative genomics, and targeted genome modification for important trait improvement of B. tulda.

## Full-text entities

- **Chemicals:** propidium iodide (MESH:D011419), agarose (MESH:D012685), FL3-A (-)
- **Species:** Ampelocalamus luodianensis [taxon 1569450], Bambusa tulda (species) [taxon 292582], Dendrocalamus latiflorus (sweet bamboo, species) [taxon 257763], Bonia amplexicaulis (species) [taxon 338503], Guadua angustifolia (Colombian timber bamboo, species) [taxon 323898], Melocanna baccifera (species) [taxon 104005], Rhipidocladum racemiflorum (species) [taxon 464972], Otatea glauca (species) [taxon 464970], Zea mays (maize, species) [taxon 4577], Raddia guianensis (species) [taxon 1482630], Bambusa (bamboos, genus) [taxon 4581], Olyra latifolia (species) [taxon 38716], Musa acuminata (banana, species) [taxon 4641], Solanum lycopersicum (tomato, species) [taxon 4081], Phyllostachys edulis (moso bamboo, species) [taxon 38705], Bambuseae (bamboo, tribe) [taxon 147376]

## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12873296/full.md

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Source: https://tomesphere.com/paper/PMC12873296