Proteomic analysis revealed the potential usefulness of faecal extracellular vesicles in colorectal cancer diagnosis

TL;DR

This study shows that proteins in faecal extracellular vesicles can help detect colorectal cancer, offering a new diagnostic approach.

Contribution

The study identifies faecal extracellular vesicles as a novel source of biomarkers for colorectal cancer diagnosis.

Findings

Proteins in faecal extracellular vesicles are enriched in colon-derived proteins, unlike those in faecal suspensions.

OLFM4 and LGALS3BP were significantly increased in colorectal cancer patients' faecal extracellular vesicles.

Faecal extracellular vesicles are a promising resource for diagnosing colorectal diseases.

Abstract

Colorectal cancer causes many deaths annually worldwide. We aimed to develop a new diagnostic method for colorectal cancer by analysing the protein profiles of extracellular vesicles in faeces. Enrichment analysis of proteomic data on extracellular vesicles highly purified from faeces by density gradient centrifugation revealed that proteins detected only in extracellular vesicles were significantly enriched in proteins derived from the colon. Notably, proteins detected only in faecal suspensions showed no enrichment in colon-derived proteins, suggesting that faecal extracellular vesicles can be used to detect changes in the protein profile that occur in the colon. In addition, we identified four candidate biomarkers among the proteins that were altered in the faecal extracellular vesicles of patients with colorectal cancer identified by proteomic analysis. By comparing different healthy control samples, olfactomedin-4 (OLFM4) and galectin-3-binding protein (LGALS3BP) in faecal extracellular vesicles were significantly increased in patients with colorectal cancer. This study shows that faecal extracellular vesicles are a promising biomarker resource for colorectal diseases, including colorectal cancers. Further analysis using faecal extracellular vesicles may lead to the development of novel diagnostic methods for colorectal cancer.

The online version contains supplementary material available at 10.1038/s41598-026-35255-5.

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