Providing maceration protocols for xylem and phloem research
Eunice Romero, Mark Olson, Alí Segovia-Rivas, Jarmila Pittermann, Radek Jupa
TL;DR
This paper provides detailed maceration protocols for studying plant vascular tissues, using safer chemicals and techniques suitable for various microscopy methods.
Contribution
The paper introduces flexible, detailed maceration protocols using Franklin’s solution for studying xylem and phloem in diverse plant species.
Findings
P1 allows non-permanent slide preparation for observing xylem and phloem under multiple microscopy techniques.
P2 and P3 produce permanent slides, with P2 effective for over 34 plant families and P3 requiring minimal substances and time.
The protocols enhance the applicability of maceration for ecological and evolutionary research.
Abstract
Maceration technique allows isolating and studying the structure of plant cells, providing essential insights into plant physiology and responses to environmental factors. Despite its importance, the methods sections of publications often lack sufficient detail to properly apply maceration technique, preventing broader applications beyond industrial and wood identification studies. Here we describe maceration protocols (P), as guidelines, to allow successful cell separation, in woody and herbaceous plants, for further studying the structure of vascular tissues (xylem and phloem), using Franklin’s solution instead of the traditional, more toxic Jeffrey’s. We included sample preparation, equipment and chemicals, recommended stains and means of slide preparation that ensure clear observation and imaging, and identified potential pitfalls and safety practices. P1 provides a simple xylem and…
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Taxonomy
TopicsPolysaccharides and Plant Cell Walls · Plant Molecular Biology Research · Plant Gene Expression Analysis