# Multicenter evaluation of the QuickMIC® rapid AST system in clinical practice: impact on turnaround time compared to routine AST systems

## TL;DR

This study compares a new rapid antibiotic susceptibility test, QuickMIC, with traditional systems, showing it provides faster results while maintaining accuracy.

## Contribution

The study demonstrates that the QuickMIC system significantly reduces time-to-result and turnaround time for antibiotic susceptibility testing in clinical practice.

## Key findings

- QuickMIC's time-to-result was 3 hours and 4 minutes, much faster than 9–19 hours for routine systems.
- QuickMIC's turnaround time was 10–11 hours 30 minutes, compared to 22–45 hours for traditional systems.
- The system showed >95% agreement and acceptable bias compared to established AST systems.

## Abstract

Increased antibiotic resistance highlights the need for new, rapid antibiotic susceptibility tests to guide therapy, especially in critical disease such as bloodstream infections and sepsis. This study investigates a new ultra-rapid AST system in multiple clinical laboratories, with respect to accuracy, speed and turnaround time in comparison to commonly used AST systems. The QuickMIC system is compared to the commonly used automated AST systems BD Phoenix™ (BD, USA), MicroScan WalkAway plus (Beckman Coulter, USA) and VITEK® 2 (bioMérieux, France) by concurrent testing of incoming positive blood-cultures with Gram-negative bacteria in four clinical laboratories located in the EU and USA, on the basis of agreement of results, time-to-result (TTR, analysis time) and turnaround time (TAT, time from blood culture positivity or blood culture processing to actionable result). A total of 155 patient samples were included, totaling 10 species of Gram-negative bacteria. The overall EA and CA between QuickMIC® GN and each routine AST system was > 95%, while overall bias was within the acceptable range of ± 30%. QuickMIC time-to-result was on average 3 h and 4 min, compared to 9–19 h for the routine systems. The average QuickMIC system turnaround-time ranged from 10 to 11 h 30 min, compared to 22–45 h for the routine systems. The QuickMIC system represents a promising rapid AST technology with potential to reduce time-to-result and overall turnaround-time of clinically actionable AST results compared with the most common routinely used automated AST methods, while maintaining good accuracy and quality of results.

The online version contains supplementary material available at 10.1007/s10096-025-05298-z.